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Biological Small Angle Scattering: Techniques, Strategies and Tips

  • Book
  • © 2017

Overview

Editors:
  1. Barnali Chaudhuri

    1. G N Ramachandran Protein Center, CSIR Institute of Microbial Technology, Chandigarh, India

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  2. Inés G. Muñoz

    1. Structural Biology Programme, Spanish National Cancer Research Centre (CNIO), Madrid, Spain

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  3. Shuo Qian

    1. Center for Structural Molecular Biology and Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, USA

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  4. Volker S. Urban

    1. Center for Structural Molecular Biology and Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, USA

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  • Includes the principles and theoretical background of various SAS techniques
  • States practical aspects that range from sample preparation to data publication
  • Covers techniques for improving data quality and analysis, as well as different scientific applications of SAS.
  • Gives abundant illustrations and practical tips
  • Explains clearly the principles and the reviews on the latest progresses and serves as a guide through all aspects of biological solution SAS for readers

Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 1009)

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Table of contents (15 chapters)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Small Angle Scattering: Historical Perspective and Future Outlook

    • Thomas M. Weiss
    Pages 1-10

  3. Sample and Buffer Preparation for SAXS

    • Melissa A. Graewert, Cy M. Jeffries
    Pages 11-30

  4. Considerations for Sample Preparation Using Size-Exclusion Chromatography for Home and Synchrotron Sources

    • Robert P. Rambo
    Pages 31-45Open Access

  5. How to Analyze and Present SAS Data for Publication

    • Martha Brennich, Petra Pernot, Adam Round
    Pages 47-64

  6. Designing and Performing Biological Solution Small-Angle Neutron Scattering Contrast Variation Experiments on Multi-component Assemblies

    • Susan Krueger
    Pages 65-85

  7. SAS-Based Structural Modelling and Model Validation

    • Maxim V. Petoukhov, Anne Tuukkanen
    Pages 87-105

  8. Structural Characterization of Highly Flexible Proteins by Small-Angle Scattering

    • Tiago N. Cordeiro, Fátima Herranz-Trillo, Annika Urbanek, Alejandro Estaña, Juan Cortés, Nathalie Sibille et al.
    Pages 107-129

  9. What Can We Learn from Wide-Angle Solution Scattering?

    • Yujing Wang, Hao Zhou, Emre Onuk, John Badger, Lee Makowski
    Pages 131-147

  10. SAS-Based Studies of Protein Fibrillation

    • Carlotta Marasini, Bente Vestergaard
    Pages 149-165

  11. High Resolution Distance Distributions Determined by X-Ray and Neutron Scattering

    • Henry Y. H. Tang, John A. Tainer, Greg L. Hura
    Pages 167-181

  12. A Successful Combination: Coupling SE-HPLC with SAXS

    • Javier Pérez, Patrice Vachette
    Pages 183-199

  13. Applications of SANS to Study Membrane Protein Systems

    • Frank Gabel
    Pages 201-214

  14. Hybrid Applications of Solution Scattering to Aid Structural Biology

    • Alexander V. Grishaev
    Pages 215-227

  15. A Practical Guide to iSPOT Modeling: An Integrative Structural Biology Platform

    • An Hsieh, Lanyuan Lu, Mark R. Chance, Sichun Yang
    Pages 229-238

  16. Small Angle Scattering for Pharmaceutical Applications: From Drugs to Drug Delivery Systems

    • Aaron Alford, Veronika Kozlovskaya, Eugenia Kharlampieva
    Pages 239-262

  17. Back Matter

    Pages 263-268
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Keywords

About this book

This book provides a clear, comprehensible and up-to-date description of how Small Angle Scattering (SAS) can help structural biology researchers. SAS is an efficient technique that offers structural information on how biological macromolecules behave in solution. SAS provides distinct and complementary data for integrative structural biology approaches in combination with other widely used probes, such as X-ray crystallography, Nuclear magnetic resonance, Mass spectrometry and Cryo-electron Microscopy. The development of brilliant synchrotron small-angle X-ray scattering (SAXS) beam lines has increased the number of researchers interested in solution scattering. SAS is especially useful for studying conformational changes in proteins, highly flexible proteins, and intrinsically disordered proteins. Small-angle neutron scattering (SANS) with neutron contrast variation is ideally suited for studying multi-component assemblies as well as membrane proteins that are stabilized in surfactant micelles or vesicles. SAS is also used for studying dynamic processes of protein fibrillation in amyloid diseases, and pharmaceutical drug delivery. The combination with size-exclusion chromatography further increases the range of SAS applications.

The book is written by leading experts in solution SAS methodologies. The principles and theoretical background of various SAS techniques are included, along with practical aspects that range from sample preparation to data presentation for publication. Topics covered include techniques for improving data quality and analysis, as well as different scientific applications of SAS. With abundant illustrations and practical tips, we hope the clear explanations of the principles and the reviews on the latest progresses will serve as a guide through all aspects of biological solution SAS.

The scope of this book is particularly relevant for structural biology researchers who are new to SAS. Advanced users of the technique will findit helpful for exploring the diversity of solution SAS methods and applications.

Chapter 3 of this book is available open access under a CC BY 4.0 license at link.springer.com.

Editors and Affiliations

  • G N Ramachandran Protein Center, CSIR Institute of Microbial Technology, Chandigarh, India

    Barnali Chaudhuri

  • Structural Biology Programme, Spanish National Cancer Research Centre (CNIO), Madrid, Spain

    Inés G. Muñoz

  • Center for Structural Molecular Biology and Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, USA

    Shuo Qian, Volker S. Urban

Bibliographic Information

  • Book Title: Biological Small Angle Scattering: Techniques, Strategies and Tips

  • Editors: Barnali Chaudhuri, Inés G. Muñoz, Shuo Qian, Volker S. Urban

  • Series Title: Advances in Experimental Medicine and Biology

  • DOI: https://doi.org/10.1007/978-981-10-6038-0

  • Publisher: Springer Singapore

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: Springer Nature Singapore Pte Ltd. 2017

  • Hardcover ISBN: 978-981-10-6037-3Published: 29 December 2017

  • Softcover ISBN: 978-981-13-5555-4Published: 11 December 2018

  • eBook ISBN: 978-981-10-6038-0Published: 07 December 2017

  • Series ISSN: 0065-2598

  • Series E-ISSN: 2214-8019

  • Edition Number: 1

  • Number of Pages: X, 268

  • Number of Illustrations: 10 b/w illustrations, 77 illustrations in colour

  • Topics: Protein Structure, Molecular Medicine, Biotechnology

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