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Hybrid Applications of Solution Scattering to Aid Structural Biology

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Biological Small Angle Scattering: Techniques, Strategies and Tips

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1009))

Abstract

Biomolecular applications of solution X-ray and neutron scattering (SAXS and SANS, respectively) started in late 1960s – early 1970s but were relatively limited in their ability to provide a detailed structural picture and lagged behind what became the two primary methods of experimental structural biology − X-ray crystallography and NMR. However, improvements in both data analysis and instrumentation led to an explosive growth in the number of studies that used small-angle scattering (SAS) for investigation of macromolecular structure, often in combination with other biophysical techniques. Such hybrid applications are nowadays quickly becoming a norm whenever scattering data are used for two reasons. First, it is generally accepted that SAS data on their own cannot lead to a uniquely defined high-resolution structural model, creating a need for supplementing them with information from complementary techniques. Second, solution scattering data are frequently applied in situations when a method such NMR or X-ray crystallography cannot provide a satisfactory structural picture, which makes these additional restraints highly desirable. Maturation of the hybrid bio-SAS approaches brings to light new questions including completeness of the conformational space sampling, model validation, and data compatibility.

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Author information

Authors and Affiliations

  1. National Institute of Standards and Technology, The Institute for Bioscience and Biotechnology Research, 9600 Gudelsky Drive, Rockville, MD, 20850, USA

    Alexander V. Grishaev

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  1. Alexander V. Grishaev
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Correspondence to Alexander V. Grishaev .

Editor information

Editors and Affiliations

  1. G N Ramachandran Protein Center, CSIR Institute of Microbial Technology, Chandigarh, India

    Barnali Chaudhuri

  2. Structural Biology Programme, Spanish National Cancer Research Centre (CNIO), Madrid, Spain

    Inés G. Muñoz

  3. Center for Structural Molecular Biology and Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

    Shuo Qian

  4. Center for Structural Molecular Biology and Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

    Volker S. Urban

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Grishaev, A.V. (2017). Hybrid Applications of Solution Scattering to Aid Structural Biology. In: Chaudhuri, B., Muñoz, I., Qian, S., Urban, V. (eds) Biological Small Angle Scattering: Techniques, Strategies and Tips. Advances in Experimental Medicine and Biology, vol 1009. Springer, Singapore. https://doi.org/10.1007/978-981-10-6038-0_13

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