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Diffusion Chamber Culture

Hemopoiesis, Cloning of Tumors, Cytogenetic and Carcinogenic Assays

  • Conference proceedings
  • © 1980

Overview

Editors:
  1. Eugene P. Cronkite

    1. Medical Department, Brookhaven National Laboratory, Upton, USA

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  2. Arland L. Carsten

    1. Medical Department, Brookhaven National Laboratory, Upton, USA

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Table of contents (24 papers)

  1. Front Matter

    Pages I-XIII
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  2. Characterization of Progenitor Cells in Diffusion Chambers

    1. Front Matter

      Pages 1-1
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    2. History and Overview of the In Vivo Diffusion Chamber (DC) Culture System

      • A. L. Carsten
      Pages 3-10

    3. Roles of CFU-S and CFU-C in Maintaining Cell Growth in Diffusion Chamber Cultures of Murine Marrow

      • L. N. Shulman, S. H. Robinson
      Pages 11-18

    4. Different Classes of Granulocyte Progenitor Cells Expressed in In Vivo Plasma Clot Diffusion Chamber Cultures

      • H. N. Steinberg, S. H. Robinson
      Pages 19-27

    5. Human Granulocytopoietic Progenitor Cell Diversity and Regulation Studied in Diffusion Chambers

      • N. Jacobsen
      Pages 28-43

    6. Studies on the Regulation of Diffusion Chamber Granulopoiesis

      • P. Quesenberry, R. Sullivan, A. Fountebouni, L. Levitt, E. Niskanen, M. Symann et al.
      Pages 44-56

    7. Megakaryocyte Growth in In Vitro Cultures and in Diffusion Chambers

      • J. M. Paulus, A. L. Carsten, E. P. Cronkite, N. Lacaze
      Pages 57-61

  3. Regulatory Mechanisms in Diffusion Chamber Cell Growth

    1. Front Matter

      Pages 63-63
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    2. Culture of Normal Human Blood Cells in a Diffusion Chamber System II. Lymphocyte and Plasma Cell Kinetics

      • G. Chikkappa, A. L. Carsten, A. D. Chanana, E. P. Cronkite
      Pages 65-71

    3. Marrow Culture in Diffusion Chambers in Rabbits IV. Effects of Leukocyte Products on Granulopoiesis

      • R. Willemze, R. I. Walker, J. L. M. Waayer, J. C. Herion, J. G. Palmer
      Pages 72-79

    4. Host-Mediated Regulatory Activities as Measured on Bone Marrow Cells Cultured in Diffusion Chambers and In Vitro

      • D. Løvhaug, A. Bøyum
      Pages 80-94

    5. Growth of Canine Bone Marrow and Peripheral Blood Mononuclear Cells in In Vivo Plasma Clot Diffusion Chambers in Mice: Cytopoietic Activity, GM-CFC Content, and Physical Separation

      • T. J. McVittie, K. F. McCarthy
      Pages 95-109

    6. Normal and Leukemic Hematopoiesis in Diffusion Chambers: Comparison with In Vitro Culture Systems

      • H. E. Broxmeyer, L. Koltun, N. Jacobsen, M. A. S. Moore
      Pages 110-123

    7. Stimulating Factor(s) of Hematopoiesis in the Bone Marrow of Mice Administered Hydroxyurea. Effects of Diffusion Chamber Progenitor Cells (DCPC) and Observations on Pluripotent Stem Cells (CFU-S)

      • Z. Ben-Ishay, S. Sharon, A. Shorr
      Pages 124-135

  4. Cell Interactions, Cytogenetics, and Methodology

    1. Front Matter

      Pages 137-137
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    2. Use of Diffusion Chambers for Studying Cell Interactions: Sequential Studies of Eosinophil Granulocytopoiesis In Vivo

      • A. M. Miller, M. P. McGarry
      Pages 139-149

    3. An Evaluation of Host Cell Contact in the Regulation of Diffusion Chamber Hematopoiesis

      • R. I. Walker, J. C. Herion, F. G. Dalldorf, R. Willemze, G. Palmer
      Pages 150-156

    4. Stem Cells in Human Peripheral Blood: A Method for Histologic Analysis of Colony Formation in Plasma Clot Diffusion Chambers

      • F. E. Zwaan, J. te Velde, A. Puliga, H. Goselink, R. Lobatto
      Pages 157-176

    5. The Growth of Colonies of Human Bone Marrow and Tumour Cells in Soft Agar in Diffusion Chambers

      • P. J. Selby, I. E. Smith, M. Y. Gordon
      Pages 177-187
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Keywords

About this book

Even though diffusion chamber culture was commenced long before ortho­ dox tissue culture by Metchnikoff (1887) there have been only sporadic attempts to use this methodology to study cell proliferation (review by Carsten, Chap. 1). Not so long ago diffusion chamber culture was nicknamed "confusion chamber" culture. I believe this conference has removed the confusion and will truly point out the intrinsic value of the system. It is not a substitute for established in vitro culture nor for in vivo studies. It complements both. Dr. Arne B~yum introduced diffusion chamber culture at Brookhaven National Laboratory. After some modest success in showing that one could culture human bone marrow and with appropriate stimuli induce erythro­ poiesis in diffusion chambers, several of the participants at this conference visited Brookhaven to learn firsthand this simple technology and to apply it in their own laboratories. However, the technique did not spread widely and controversy arose in which the same question was repeatedly asked: Can the diffusion chamber technique provide information that is not ob­ tainable more rapidly and easily, and at less expense by the in vitro tech­ niques? As a result of our deep interest in and involvement with diffusion chamber culture Dr. A. L. Carsten and I organized this conference. A major objective of this conference was to seek answers to the above question.

Editors and Affiliations

  • Medical Department, Brookhaven National Laboratory, Upton, USA

    Eugene P. Cronkite, Arland L. Carsten

Bibliographic Information

  • Book Title: Diffusion Chamber Culture

  • Book Subtitle: Hemopoiesis, Cloning of Tumors, Cytogenetic and Carcinogenic Assays

  • Editors: Eugene P. Cronkite, Arland L. Carsten

  • DOI: https://doi.org/10.1007/978-3-642-67644-4

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1980

  • Softcover ISBN: 978-3-540-10064-5Published: 01 September 1980

  • eBook ISBN: 978-3-642-67644-4Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XIV, 277

  • Number of Illustrations: 12 b/w illustrations

  • Topics: Oncology

  • Industry Sectors: Biotechnology, Health & Hospitals, Pharma

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