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Human Granulocytopoietic Progenitor Cell Diversity and Regulation Studied in Diffusion Chambers

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Diffusion Chamber Culture

Abstract

In normal human bone marrow, three different populations of progenitor cells capable of giving rise to neutrophilic granulocytes can be distinguished. CFU-D which form neutrophilic colonies in diffusion chambers in irradiated mice can be separated from day 14 CFU-C and day 7 CFU-C which form colonies in agar cultures in vitro in response to leukocyte-derived colony stimulating activity (CSA). Kinetic studies suggest that these cells are serially connected in a maturation sequence in that order. In normal marrow, the incidence of the cells and the mitotic turnover rate increase with the degree of maturity. Furthermore, the maturation of progenitor cells may be associated with an increasing responsiveness to CSA. All three cell types are stimulated by species nonspecific diffusible factors in irradiated mice (or inhibited by factors in nonirradiated mice). These studies suggest that an analogy exists between the organization of erythroid and neutrophilic progenitor cells.

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Authors
  1. N. Jacobsen
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Editors and Affiliations

  1. Medical Department, Brookhaven National Laboratory, Upton, N.Y., USA

    Eugene P. Cronkite MD  & Arland L. Carsten PhD  & 

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© 1980 Springer-Verlag Berlin Heidelberg

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Jacobsen, N. (1980). Human Granulocytopoietic Progenitor Cell Diversity and Regulation Studied in Diffusion Chambers. In: Cronkite, E.P., Carsten, A.L. (eds) Diffusion Chamber Culture. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67644-4_4

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  • DOI: https://doi.org/10.1007/978-3-642-67644-4_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-10064-5

  • Online ISBN: 978-3-642-67644-4

  • eBook Packages: Springer Book Archive

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