Overview
- Offers modern approaches to generate high quality monoclonals
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1131)
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Table of contents (36 protocols)
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Antibody Generation
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Antibody Expression and Purification
Keywords
- aggregated monoclonals
- antigen generation
- crystallization chaperoning
- downstream purification of IgG
- immunofluorescence
- monoclonal antibodies
- monoclonal antibody characterization
- monoclonal antibody expression
- monoclonal antibody generation
- monoclonal antibody modification
- monoclonal antibody purification
- mutagenesis
- phage- or bacterial display
- solid-state arrays
- unstable monoclonals
- antibodies
About this book
Monoclonal Antibodies: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen generation to epitope mapping and quality control of the purified IgG. Chapters are divided into four parts corresponding to four distinct objectives. Part I covers monoclonal antibody generation, Part II deals with monoclonal antibody expression and purification, Part III presents methods for monoclonal antibody characterization and modification, and Part IV describes selected applications of monoclonal antibodies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Monoclonal Antibodies: Methods and Protocols, Second Edition provides crucial initial steps of monoclonal antibody generation and characterization with state-of-the art protocols.
Editors and Affiliations
Bibliographic Information
Book Title: Monoclonal Antibodies
Book Subtitle: Methods and Protocols
Editors: Vincent Ossipow, Nicolas Fischer
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-992-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, New York 2014
Hardcover ISBN: 978-1-62703-991-8Published: 12 February 2014
Softcover ISBN: 978-1-4939-6322-5Published: 23 August 2016
eBook ISBN: 978-1-62703-992-5Published: 10 February 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIV, 575
Number of Illustrations: 81 b/w illustrations, 27 illustrations in colour
Topics: Immunology, Laboratory Medicine, Antibodies
Industry Sectors: Biotechnology, Consumer Packaged Goods, Health & Hospitals, Pharma