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Adeno-Associated Virus

Methods and Protocols

  • Richard O. Snyder
  • Philippe Moullier

Part of the Methods in Molecular Biology book series (MIMB, volume 807)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Matthew D. Weitzman, R. Michael Linden
    Pages 1-23
  3. John T. Gray, Serge Zolotukhin
    Pages 25-46
  4. Mavis Agbandje-McKenna, Jürgen Kleinschmidt
    Pages 47-92
  5. Chunping Qiao, Taeyoung Koo, Juan Li, Xiao Xiao, J. George Dickson
    Pages 119-140
  6. Mark S. Sands
    Pages 141-157
  7. Olivier Bockstael, Kevin D. Foust, Brian Kaspar, Liliane Tenenbaum
    Pages 159-177
  8. Knut Stieger, Therese Cronin, Jean Bennett, Fabienne Rolling
    Pages 179-218
  9. Lawrence T. Bish, H. Lee Sweeney, Oliver J. Müller, Raffi Bekeredjian
    Pages 219-237
  10. K. Reed Clark, Magalie Penaud-Budloo
    Pages 239-258
  11. Ashley T. Martino, Roland W. Herzog, Ignacio Anegon, Oumeya Adjali
    Pages 259-272
  12. Hildegard Büning, Chelsea M. Bolyard, Michael Hallek, Jeffrey S. Bartlett
    Pages 273-300
  13. Daniel G. Miller
    Pages 301-315
  14. Terence R. Flotte, Thomas J. Conlon, Christian Mueller
    Pages 317-337
  15. Caroline Le Guiner, Phillipe Moullier, Valder R. Arruda
    Pages 339-359
  16. Lijun Wang, Véronique Blouin, Nicole Brument, Mahajoub Bello-Roufai, Achille Francois
    Pages 361-404
  17. Richard O. Snyder, Muriel Audit, Joyce D. Francis
    Pages 405-428
  18. Katherine A. High, Patrick Aubourg
    Pages 429-457
  19. Back Matter
    Pages 459-462

About this book

Introduction

Today, progress in rAAV-mediated gene transfer is so robust that long-term, efficient, and regulatable transgene expression is reproducibly achieved in large animal models. The complexity of  gene transfer agents in the context of their clinical use requires investigators from a wide variety of backgrounds to have an understanding — or at least an appreciation of — the regulatory environment and constraints that affect vector design, manufacturing, pre-clinical testing, and clinical use, with an emphasis on patient protection. In Adeno-Associated Virus: Methods and Protocols, experts from the United States and Europe have contributed current knowledge of this multi-dimensional field relating to the biology of AAV, rAAV vector design, vector manufacturing and product testing, performance of rAAV vectors in major organs, rAAV-related immunological issues, design of animal and clinical studies, and clinical experience. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Adeno-Associated Virus: Methods and Protocols provides a complete and comprehensive understanding of this multi-disciplinary and rapidly progressing field.

Keywords

Adeno-associated virus vectors Adeno-associated virus-mediated gene targeting gene therapy recombinant adeno-associated virus recombinant adeno-associated virus gene transfer

Editors and affiliations

  • Richard O. Snyder
    • 1
  • Philippe Moullier
    • 2
  1. 1.Center of Excellence for Regenerative, Health Biotechnology (CERHB)University of FloridaAlachuaUSA
  2. 2.INSERM U649, Labo. Thérapie GéniqueCHU Hôtel DieuNantes CX 1France

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-370-7
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-369-1
  • Online ISBN 978-1-61779-370-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site