Skip to main content
SpringerLink
Log in

Production and Purification of Recombinant Adeno-Associated Vectors

  • Protocol
  • First Online:
Book cover Adeno-Associated Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 807))

Abstract

The use of recombinant adeno-associated virus (rAAV) vectors in gene therapy for preclinical studies in animal models and human clinical trials is increasing, as these vectors have been shown to be safe and to mediate persistent transgene expression in vivo. Constant improvement in rAAV manufacturing processes (upstream production and downstream purification) has paralleled this evolution to meet the needs for larger vector batches, higher vector titer, and improved vector quality and safety. This chapter provides an overview of existing production and purification systems used for adeno-associated virus (AAV) vectors, and the advantages and disadvantages of each system are outlined. Regulatory guidelines that apply to the use of these systems for clinical trials are also presented. The methods described are examples of protocols that have been utilized for establishing rAAV packaging cell lines, production of rAAV vectors using recombinant HSV infection, and for chromatographic purification of various AAV vector serotypes. A protocol for the production of clinical-grade rAAV type 2 vectors using transient transfection and centrifugation-based purification is also described.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 139.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 179.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. McLaughlin, S. K., Collis, P., Hermonat, P. L., and Muzyczka, N. (1988) Adeno-associated virus general transduction vectors: analysis of proviral structures, J Virol 62, 1963–1973.

    PubMed  CAS  Google Scholar 

  2. Salvetti, A., Oreve, S., Chadeuf, G., Favre, D., Cherel, Y., Champion-Arnaud, P., David-Ameline, J., and Moullier, P. (1998) Factors influencing recombinant adeno-associated virus production, Hum Gene Ther 9, 695–706.

    Article  PubMed  CAS  Google Scholar 

  3. Merten, O. W., Geny-Fiamma, C., and Douar, A. M. (2005) Current issues in adeno-associated viral vector production, Gene Ther 12Suppl 1, S51-61.

    Article  PubMed  CAS  Google Scholar 

  4. Aucoin, M. G., Perrier, M., and Kamen, A. A. (2008) Critical assessment of current adeno-associated viral vector production and quantification methods, Biotechnol Adv 26, 73–88.

    Article  PubMed  CAS  Google Scholar 

  5. Urabe, M., Ding, C., and Kotin, R. M. (2002) Insect cells as a factory to produce adeno-associated virus type 2 vectors, Hum Gene Ther 13, 1935–1943.

    Article  PubMed  CAS  Google Scholar 

  6. Smith, R. H., Levy, J. R., and Kotin, R. M. (2009) A simplified baculovirus-AAV expression vector system coupled with one-step affinity purification yields high-titer rAAV stocks from insect cells, Mol Ther 17, 1888–1896.

    Article  PubMed  CAS  Google Scholar 

  7. Aslanidi, G., Lamb, K., and Zolotukhin, S. (2009) An inducible system for highly efficient production of recombinant adeno-associated virus (rAAV) vectors in insect Sf9 cells, Proc Natl Acad Sci U S A 106, 5059–5064.

    Article  PubMed  CAS  Google Scholar 

  8. Xiao, X., Li, J., and Samulski, R. J. (1998) Production of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus, J Virol 72, 2224–2232.

    PubMed  CAS  Google Scholar 

  9. Rabinowitz, J. E., Rolling, F., Li, C., Conrath, H., Xiao, W., Xiao, X., and Samulski, R. J. (2002) Cross-packaging of a single adeno-associated virus (AAV) type 2 vector genome into multiple AAV serotypes enables transduction with broad specificity, J Virol 76, 791–801.

    Article  PubMed  CAS  Google Scholar 

  10. Grimm, D., Kern, A., Rittner, K., and Kleinschmidt, J. A. (1998) Novel tools for production and purification of recombinant adenoassociated virus vectors, Hum Gene Ther 9, 2745–2760.

    Article  PubMed  CAS  Google Scholar 

  11. Collaco, R. F., Cao, X., and Trempe, J. P. (1999) A helper virus-free packaging system for recombinant adeno-associated virus vectors, Gene 238, 397–405.

    Article  PubMed  CAS  Google Scholar 

  12. Grimm, D., Kay, M. A., and Kleinschmidt, J. A. (2003) Helper virus-free, optically controllable, and two-plasmid-based production of adeno-associated virus vectors of serotypes 1 to 6, Mol Ther 7, 839–850.

    Article  PubMed  CAS  Google Scholar 

  13. Gao, G. P., Qu, G., Faust, L. Z., Engdahl, R. K., Xiao, W., Hughes, J. V., Zoltick, P. W., and Wilson, J. M. (1998) High-titer adeno-associated viral vectors from a Rep/Cap cell line and hybrid shuttle virus, Hum Gene Ther 9, 2353–2362.

    Article  PubMed  CAS  Google Scholar 

  14. Liu, X. L., Clark, K. R., and Johnson, P. R. (1999) Production of recombinant adeno-associated virus vectors using a packaging cell line and a hybrid recombinant adenovirus, Gene Ther 6, 293–299.

    Article  PubMed  CAS  Google Scholar 

  15. Zhang, H., Xie, J., Xie, Q., Wilson, J. M., and Gao, G. (2009) Adenovirus-adeno-associated virus hybrid for large-scale recombinant adeno-associated virus production, Hum Gene Ther 20, 922–929.

    Article  PubMed  CAS  Google Scholar 

  16. Clark, K. R., Voulgaropoulou, F., Fraley, D. M., and Johnson, P. R. (1995) Cell lines for the production of recombinant adeno-associated virus, Hum Gene Ther 6, 1329–1341.

    Article  PubMed  CAS  Google Scholar 

  17. Blouin, V., Brument, N., Toublanc, E., Raimbaud, I., Moullier, P., and Salvetti, A. (2004) Improving rAAV production and purification: towards the definition of a scaleable process, J Gene Med 6 Suppl 1, S223-228.

    Article  PubMed  CAS  Google Scholar 

  18. Thorne, B. A., Takeya, R. K., and Peluso, R. W. (2009) Manufacturing recombinant adeno-associated viral vectors from producer cell clones, Hum Gene Ther 20, 707–714.

    Article  PubMed  CAS  Google Scholar 

  19. Toublanc, E., Benraiss, A., Bonnin, D., Blouin, V., Brument, N., Cartier, N., Epstein, A. L., Moullier, P., and Salvetti, A. (2004) Identification of a replication-defective herpes simplex virus for recombinant adeno-associated virus type 2 (rAAV2) particle assembly using stable producer cell lines, J Gene Med 6, 555–564.

    Article  PubMed  CAS  Google Scholar 

  20. Gao, G. P., Lu, F., Sanmiguel, J. C., Tran, P. T., Abbas, Z., Lynd, K. S., Marsh, J., Spinner, N. B., and Wilson, J. M. (2002) Rep/Cap gene amplification and high-yield production of AAV in an A549 cell line expressing Rep/Cap, Mol Ther 5, 644–649.

    Article  PubMed  CAS  Google Scholar 

  21. Farson, D., Harding, T. C., Tao, L., Liu, J., Powell, S., Vimal, V., Yendluri, S., Koprivnikar, K., Ho, K., Twitty, C., Husak, P., Lin, A., Snyder, R. O., and Donahue, B. A. (2004) Development and characterization of a cell line for large-scale, serum-free production of recombinant adeno-associated viral vectors, J Gene Med 6, 1369–1381.

    Article  PubMed  CAS  Google Scholar 

  22. Conway, J. E., Rhys, C. M., Zolotukhin, I., Zolotukhin, S., Muzyczka, N., Hayward, G. S., and Byrne, B. J. (1999) High-titer recombinant adeno-associated virus production utilizing a recombinant herpes simplex virus type I vector expressing AAV-2 Rep and Cap, Gene Ther 6, 986–993.

    Article  PubMed  CAS  Google Scholar 

  23. Kang, W., Wang, L., Harrell, H., Liu, J., Thomas, D. L., Mayfield, T. L., Scotti, M. M., Ye, G. J., Veres, G., and Knop, D. R. (2009) An efficient rHSV-based complementation system for the production of multiple rAAV vector serotypes, Gene Ther 16, 229–239.

    Article  PubMed  CAS  Google Scholar 

  24. Booth, M. J., Mistry, A., Li, X., Thrasher, A., and Coffin, R. S. (2004) Transfection-free and scalable recombinant AAV vector production using HSV/AAV hybrids, Gene Ther 11, 829–837.

    Article  PubMed  CAS  Google Scholar 

  25. Negrete, A., and Kotin, R. M. (2008) Large-scale production of recombinant adeno-associated viral vectors, Methods Mol Biol 433, 79–96.

    Article  PubMed  CAS  Google Scholar 

  26. Negrete, A., and Kotin, R. M. (2007) Production of recombinant adeno-associated vectors using two bioreactor configurations at different scales, J Virol Methods 145, 155–161.

    Article  PubMed  CAS  Google Scholar 

  27. Cecchini, S., Virag, T., Negrete, A., and Kotin, R. M. (2009) Production and Processing of rAAVU7smOPT in 100L Bioreactors for Canine Models of Duchenne Muscular Dystrophy, Mol Ther 17, 17.

    Google Scholar 

  28. Durocher, Y., Pham, P. L., St-Laurent, G., Jacob, D., Cass, B., Chahal, P., Lau, C. J., Nalbantoglu, J., and Kamen, A. (2007) Scalable serum-free production of recombinant adeno-associated virus type 2 by transfection of 293 suspension cells, J Virol Methods 144, 32–40.

    Article  PubMed  CAS  Google Scholar 

  29. Hauck, B., Qu, G., Zelenaia, O., Zhou, J., Liu, X., Africa, L., High, K. A., and Wright, J. F. (2009) A Scalable Manufacturing Platform for Purification of AAV Serotypes 2, 5, 6 and 8 for IND-Supporting Pre-Clinical Studies and Clinical Trials, Mol Ther 17, S17.

    Article  Google Scholar 

  30. Thomas, D. L., Wang, L., Niamke, J., Liu, J., Kang, W., Scotti, M. M., Ye, G. J., Veres, G., and Knop, D. R. (2009) Scalable recombinant adeno-associated virus production using recombinant herpes simplex virus type 1 coinfection of suspension-adapted mammalian cells, Hum Gene Ther 20, 861–870.

    Article  PubMed  CAS  Google Scholar 

  31. Zeltner, N., Kohlbrenner, E., Clément, N., Weber, T., and Linden, R. M. (2010) Near-perfect infectivity of wild-type AAV as benchmark for infectivity of recombinant AAV vectors., Gene Ther 17.

    Google Scholar 

  32. Ayuso, E., Mingozzi, F., Montane, J., Leon, X., Anguela, X. M., Haurigot, V., Edmonson, S. A., Africa, L., Zhou, S., High, K. A., Bosch, F., and Wright, J. F. (2009) High AAV vector purity results in serotype- and tissue-independent enhancement of transduction efficiency, Gene Ther.

    Google Scholar 

  33. Wright, J. F. (2009) Transient transfection methods for clinical adeno-associated viral vector production, Hum Gene Ther 20, 698–706.

    Article  PubMed  CAS  Google Scholar 

  34. Knop, D. R., and Harrell, H. (2007) Bioreactor production of recombinant herpes simplex virus vectors, Biotechnol Prog 23, 715–721.

    Article  PubMed  CAS  Google Scholar 

  35. Virag, T., Cecchini, S., and Kotin, R. M. (2009) Producing recombinant adeno-associated virus in foster cells: overcoming production limitations using a baculovirus-insect cell expression strategy, Hum Gene Ther 20, 807–817.

    Article  PubMed  CAS  Google Scholar 

  36. Pijlman, G. P., van Schijndel, J. E., and Vlak, J. M. (2003) Spontaneous excision of BAC vector sequences from bacmid-derived baculovirus expression vectors upon passage in insect cells, J Gen Virol 84, 2669–2678.

    Article  PubMed  CAS  Google Scholar 

  37. Edelstein, M. L., Abedi, M. R., and Wixon, J. (2007) Gene therapy clinical trials worldwide to 2007--an update, J Gene Med 9, 833–842.

    Article  PubMed  Google Scholar 

  38. Gao, G., Qu, G., Burnham, M. S., Huang, J., Chirmule, N., Joshi, B., Yu, Q. C., Marsh, J. A., Conceicao, C. M., and Wilson, J. M. (2000) Purification of recombinant adeno-associated virus vectors by column chromatography and its performance in vivo, Hum Gene Ther 11, 2079–2091.

    Article  PubMed  CAS  Google Scholar 

  39. O’Riordan, C. R., Lachapelle, A. L., Vincent, K. A., and Wadsworth, S. C. (2000) Scaleable chromatographic purification process for recombinant adeno-associated virus (rAAV), J Gene Med 2, 444–454.

    Article  PubMed  Google Scholar 

  40. Brument, N., Morenweiser, R., Blouin, V., Toublanc, E., Raimbaud, I., Cherel, Y., Folliot, S., Gaden, F., Boulanger, P., Kroner-Lux, G., Moullier, P., Rolling, F., and Salvetti, A. (2002) A versatile and scalable two-step ion-exchange chromatography process for the purification of recombinant adeno-associated virus serotypes-2 and −5, Mol Ther 6, 678–686.

    Article  PubMed  CAS  Google Scholar 

  41. Kaludov, N., Handelman, B., and Chiorini, J. A. (2002) Scalable purification of adeno-associated virus type 2, 4, or 5 using ion-exchange chromatography, Hum Gene Ther 13, 1235–1243.

    Article  PubMed  CAS  Google Scholar 

  42. Davidoff, A. M., Ng, C. Y., Sleep, S., Gray, J., Azam, S., Zhao, Y., McIntosh, J. H., Karimipoor, M., and Nathwani, A. C. (2004) Purification of recombinant adeno-associated virus type 8 vectors by ion exchange chromatography generates clinical grade vector stock, J Virol Methods 121, 209–215.

    Article  PubMed  CAS  Google Scholar 

  43. Smith, R. H., Ding, C., and Kotin, R. M. (2003) Serum-free production and column purification of adeno-associated virus type 5, J Virol Methods 114, 115–124.

    Article  PubMed  CAS  Google Scholar 

  44. Chahal, P. S., Aucoin, M. G., and Kamen, A. (2007) Primary recovery and chromatographic purification of adeno-associated virus type 2 produced by baculovirus/insect cell system, J Virol Methods 139, 61–70.

    Article  PubMed  CAS  Google Scholar 

  45. Qu, G., Bahr-Davidson, J., Prado, J., Tai, A., Cataniag, F., McDonnell, J., Zhou, J., Hauck, B., Luna, J., Sommer, J. M., Smith, P., Zhou, S., Colosi, P., High, K. A., Pierce, G. F., and Wright, J. F. (2007) Separation of adeno-associated virus type 2 empty particles from genome containing vectors by anion-exchange column chromatography, J Virol Methods 140, 183–192.

    Article  PubMed  CAS  Google Scholar 

  46. Urabe, M., Xin, K. Q., Obara, Y., Nakakura, T., Mizukami, H., Kume, A., Okuda, K., and Ozawa, K. (2006) Removal of empty capsids from type 1 adeno-associated virus vector stocks by anion-exchange chromatography potentiates transgene expression, Mol Ther 13, 823–828.

    Article  PubMed  CAS  Google Scholar 

  47. Tamayose, K., Hirai, Y., and Shimada, T. (1996) A new strategy for large-scale preparation of high-titer recombinant adeno-associated virus vectors by using packaging cell lines and sulfonated cellulose column chromatography, Hum Gene Ther 7, 507–513.

    Article  PubMed  CAS  Google Scholar 

  48. Francis, J. D. a. S., R.O. (2005) Production of Research and Clinical Grade Recombinant Adeno-associated Viral Vectors., Vol. 31, Elsevier, Amsterdam.

    Google Scholar 

  49. Okada, T., Nonaka-Sarukawa, M., Uchibori, R., Kinoshita, K., Hayashita-Kinoh, H., Nitahara-Kasahara, Y., Takeda, S., and Ozawa, K. (2009) Scalable purification of adeno-associated virus serotype 1 (AAV1) and AAV8 vectors, using dual ion-exchange adsorptive membranes, Hum Gene Ther 20, 1013–1021.

    Article  PubMed  CAS  Google Scholar 

  50. Auricchio, A., Hildinger, M., O’Connor, E., Gao, G. P., and Wilson, J. M. (2001) Isolation of highly infectious and pure adeno-associated virus type 2 vectors with a single-step gravity-flow column, Hum Gene Ther 12, 71–76.

    Article  PubMed  CAS  Google Scholar 

  51. Snyder, R. O., and Flotte, T. R. (2002) Production of clinical-grade recombinant adeno-associated virus vectors, Curr Opin Biotechnol 13, 418–423.

    Article  PubMed  CAS  Google Scholar 

  52. Clark, K. R., Liu, X., McGrath, J. P., and Johnson, P. R. (1999) Highly purified recombinant adeno-associated virus vectors are biologically active and free of detectable helper and wild-type viruses, Hum Gene Ther 10, 1031–1039.

    Article  PubMed  CAS  Google Scholar 

  53. Anderson, R., Macdonald, I., Corbett, T., Whiteway, A., and Prentice, H. G. (2000) A method for the preparation of highly purified adeno-associated virus using affinity column chromatography, protease digestion and solvent extraction, J Virol Methods 85, 23–34.

    Article  PubMed  CAS  Google Scholar 

  54. Auricchio, A., O’Connor, E., Hildinger, M., and Wilson, J. M. (2001) A single-step affinity column for purification of serotype-5 based adeno-associated viral vectors, Mol Ther 4, 372–374.

    Article  PubMed  CAS  Google Scholar 

  55. Cecchini, S., Negrete, A., and Kotin, R. M. (2008) Toward exascale production of recombinant adeno-associated virus for gene transfer applications, Gene Ther 15, 823–830.

    Article  PubMed  CAS  Google Scholar 

  56. Wang, L., and Knop, D. R. (2007) Affinity Chromatography Purification of rAAV Vectors Produced by Transfection or rHSV Co-infection Using CaptureSelect AAV ligand, Mol Ther 15.

    Google Scholar 

  57. Wang, L., Niamke, J., Veres, G., and Knop, D. (2008) Two-Step Scalable Purification Process of rAAV1 Vectors Produced by rHSV Co-Infection in Suspension BHK Cells, Mol Ther 16, 291.

    Google Scholar 

  58. Wang, L., Veres, G., and Knop, D. R. (2009) Two-step Chromatography Purification of rAAV1 Vectors produced by Suspension BHK Cells rHSV Co-infection, Mol Ther 17, 715.

    Google Scholar 

  59. Arnold, G. S., Sasser, A. K., Stachler, M. D., and Bartlett, J. S. (2006) Metabolic biotinylation provides a unique platform for the purification and targeting of multiple AAV vector serotypes, Mol Ther 14, 97–106.

    Article  PubMed  CAS  Google Scholar 

  60. Stachler, M. D., and Bartlett, J. S. (2006) Mosaic vectors comprised of modified AAV1 capsid proteins for efficient vector purification and targeting to vascular endothelial cells, Gene Ther 13, 926–931.

    PubMed  CAS  Google Scholar 

  61. Koerber, J. T., Jang, J. H., Yu, J. H., Kane, R. S., and Schaffer, D. V. (2007) Engineering adeno-associated virus for one-step purification via immobilized metal affinity chromatography, Hum Gene Ther 18, 367–378.

    Article  PubMed  CAS  Google Scholar 

  62. Smith, R. H., Yang, L., and Kotin, R. M. (2008) Chromatography-based purification of adeno-associated virus, Methods Mol Biol 434, 37–54.

    Article  PubMed  CAS  Google Scholar 

  63. Zolotukhin, S., Byrne, B. J., Mason, E., Zolotukhin, I., Potter, M., Chesnut, K., Summerford, C., Samulski, R. J., and Muzyczka, N. (1999) Recombinant adeno-associated virus purification using novel methods improves infectious titer and yield, Gene Ther 6, 973–985.

    Article  PubMed  CAS  Google Scholar 

  64. Summerford, C., and Samulski, R. J. (1998) Membrane-associated heparan sulfate proteoglycan is a receptor for adeno-associated virus type 2 virions, J Virol 72, 1438–1445.

    PubMed  CAS  Google Scholar 

  65. Mizukami, H., Young, N. S., and Brown, K. E. (1996) Adeno-associated virus type 2 binds to a 150-kilodalton cell membrane glycoprotein, Virology 217, 124–130.

    Article  PubMed  CAS  Google Scholar 

  66. Qiu, J., Handa, A., Kirby, M., and Brown, K. E. (2000) The interaction of heparin sulfate and adeno-associated virus 2, Virology 269, 137–147.

    Article  PubMed  CAS  Google Scholar 

  67. Kaludov, N., Brown, K. E., Walters, R. W., Zabner, J., and Chiorini, J. A. (2001) Adeno-associated virus serotype 4 (AAV4) and AAV5 both require sialic acid binding for hemagglutination and efficient transduction but differ in sialic acid linkage specificity, J Virol 75, 6884–6893.

    Article  PubMed  CAS  Google Scholar 

  68. Snyder, R. O., and Francis, J. (2005) Adeno-associated viral vectors for clinical gene transfer studies, Curr Gene Ther 5, 311–321.

    Article  PubMed  CAS  Google Scholar 

  69. Mandel, R. J., Burger, C., and Snyder, R. O. (2008) Viral vectors for in vivo gene transfer in Parkinson’s disease: properties and clinical grade production, Exp Neurol 209, 58–71.

    Article  PubMed  CAS  Google Scholar 

  70. Chadeuf, G., Favre, D., Tessier, J., Provost, N., Nony, P., Kleinschmidt, J., Moullier, P., and Salvetti, A. (2000) Efficient recombinant adeno-associated virus production by a stable rep-cap HeLa cell line correlates with adenovirus-induced amplification of the integrated rep-cap genome, J Gene Med 2, 260–268.

    Article  PubMed  CAS  Google Scholar 

  71. Mathews, L. C., Gray, J.T., Gallagher, M.R., and Snyder, R.O. (2002) Recombinant Adeno-associated Viral Vector Production Using Stable Packaging and Producer Cell Lines., in Methods in Enzymology, pp 393–413.

    Google Scholar 

  72. Nony, P., Chadeuf, G., Tessier, J., Moullier, P., and Salvetti, A. (2003) Evidence for packaging of rep-cap sequences into adeno-associated virus (AAV) type 2 capsids in the absence of inverted terminal repeats: a model for generation of rep-positive AAV particles, J Virol 77, 776–781.

    Article  PubMed  CAS  Google Scholar 

  73. Francois, A., Guilbaud, M., Awedikian, R., Chadeuf, G., Moullier, P., and Salvetti, A. (2005) The cellular TATA binding protein is required for rep-dependent replication of a minimal adeno-associated virus type 2 p5 element, J Virol 79, 11082–11094.

    Article  PubMed  CAS  Google Scholar 

  74. Rice, S. A., and Knipe, D. M. (1990) Genetic evidence for two distinct transactivation functions of the herpes simplex virus alpha protein ICP27, J Virol 64, 1704–1715.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Center of Excellence for Regenerative Health Biotechnology, University of Florida, Alachua, FL, USA

    Lijun Wang & Mahajoub Bello-Roufai

  2. Laboratoire de Thérapie Génique, INSERM, Université de Nantes, Nantes, France

    Véronique Blouin & Achille Francois

  3. INSERM UMR649, Institut de Recherche Thérapeutique - IRT1, Université de Nantes, Nantes, Cedex 01, France

    Nicole Brument

Authors
  1. Lijun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Véronique Blouin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nicole Brument
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mahajoub Bello-Roufai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Achille Francois
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Achille Francois .

Editor information

Editors and Affiliations

  1. Center of Excellence for Regenerative, Health Biotechnology (CERHB), University of Florida, Innovation Drive 13706, Alachua, 32615, Florida, USA

    Richard O. Snyder

  2. INSERM U649, Labo. Thérapie Génique, CHU Hôtel Dieu, bd. Jean Monnet 30, Nantes CX 1, 44035, France

    Philippe Moullier

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Wang, L., Blouin, V., Brument, N., Bello-Roufai, M., Francois, A. (2012). Production and Purification of Recombinant Adeno-Associated Vectors. In: Snyder, R., Moullier, P. (eds) Adeno-Associated Virus. Methods in Molecular Biology, vol 807. Humana Press. https://doi.org/10.1007/978-1-61779-370-7_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-370-7_16

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-369-1

  • Online ISBN: 978-1-61779-370-7

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation