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Adeno-Associated Virus Biology

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Adeno-Associated Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 807))

Abstract

Adeno-associated virus (AAV) was first discovered as a contaminant of adenovirus stocks in the 1960s. The development of recombinant AAV vectors (rAAV) was facilitated by early studies that generated infectious molecular clones, determined the sequence of the genome, and defined the genetic elements of the virus. The refinement of methods and protocols for the production and application of rAAV vectors has come from years of studies that explored the basic biology of this virus and its interaction with host cells. Interest in improving vector performance has in turn driven studies that have provided tremendous insights into the basic biology of the AAV lifecycle. In this chapter, we review the background on AAV biology and its exploitation for vectors and gene delivery.

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Acknowledgments

This article is an overview of AAV basic biology and is not an exhaustive review of the topic. We apologize for primary work not covered here either from lack of space or inadvertent oversight. Work on AAV in the Weitzman laboratory has been supported by NIH grants (AI067952, CA097093, AI051686, and AI074967) and a Pioneer Developmental Chair from the Salk Institute. AAV work in the Linden laboratory was supported by NIH grants GM07390, 1GM071023, and GM075019, and by King’s College London School of Medicine.

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  1. Salk Institute, La Jolla, CA, USA

    Matthew D. Weitzman

  2. King’s College London School of Medicine, London, UK

    R. Michael Linden

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  1. Matthew D. Weitzman
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  2. R. Michael Linden
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Correspondence to R. Michael Linden .

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  1. Center of Excellence for Regenerative, Health Biotechnology (CERHB), University of Florida, Innovation Drive 13706, Alachua, 32615, Florida, USA

    Richard O. Snyder

  2. INSERM U649, Labo. Thérapie Génique, CHU Hôtel Dieu, bd. Jean Monnet 30, Nantes CX 1, 44035, France

    Philippe Moullier

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Weitzman, M.D., Linden, R.M. (2012). Adeno-Associated Virus Biology. In: Snyder, R., Moullier, P. (eds) Adeno-Associated Virus. Methods in Molecular Biology, vol 807. Humana Press. https://doi.org/10.1007/978-1-61779-370-7_1

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