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RT-PCR Protocols

Second Edition

  • Nicola King

Part of the Methods in Molecular Biology book series (MIMB, volume 630)

Table of contents

  1. Front Matter
    Pages i-xi
  2. The RT-PCR Detective: Hunting Down the Best Method

    1. Front Matter
      Pages 1-1
    2. Melissa Oliveira-Cunha, Ajith K. Siriwardena, Richard J. Byers
      Pages 13-32
    3. Stephanie D. Fiedler, Martha Z. Carletti, Lane K. Christenson
      Pages 49-64
    4. Alejandra Castillo Alvarez, Victoria Boyd, Richard Lai, Sandy Pineda, Cheryl Bletchly, Hans G. Heine et al.
      Pages 65-81
    5. Anita Szalmás, József Kónya, István Sziklai, Tamás Karosi
      Pages 83-95
    6. Ana Silvia González-Reiche, María de Lourdes Monzón-Pineda, Barbara W. Johnson, María Eugenia Morales-Betoulle
      Pages 109-124
    7. Eric C. H. Ho, Michael E. Donaldson, Barry J. Saville
      Pages 125-138
    8. Xiaofeng Fan, Adrian M. Di Bisceglie
      Pages 139-149
  3. The RT-PCR Mathematician: Assessing Gene and RNA Expression

  4. The RT-PCR Master Chef: Finding the Right Ingredients

    1. Front Matter
      Pages 250-250
    2. Kelvin Li, Anushka Brownley
      Pages 271-299
    3. Natasha Paul, Jonathan Shum, Tony Le
      Pages 301-318
  5. Back Matter
    Pages 337-341

About this book

Introduction

Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.

Keywords

Cloning Gene sequences PCR Primer design Real-time quantitative RT-PCR Reverse transcription Reverse-transcription polymerase chain reaction developmental biology gene expression genes molecular biology transcription

Editors and affiliations

  • Nicola King
    • 1
  1. 1.School of Science & TechnologyUniversity of New EnglandArmidaleAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-629-0
  • Copyright Information Springer Science+Business Media, LLC 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-628-3
  • Online ISBN 978-1-60761-629-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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