Abstract
CD46 (Membrane Cofactor Protein, MCP) is a transmembrane glycoprotein, which is expressed by all nucleated human cells whose purpose is to protect against autologous complement attack. In addition, CD46 can serve as a receptor for several viruses and bacteria and as a potent regulator of the inflammatory response by affecting T cell differentiation. Multiple isoforms of CD46 exist due to alternative splicing and are coexpressed in human cells in various patterns and expression levels. However, specific diseases have not been associated with isoform coexpression. We applied a nested RT-PCR method to investigate the coexpression pattern of CD46 splicing variants in otosclerotic and normal stapes footplate specimens. Using this method, we detected an altered isoform expression pattern and identified four novel CD46 splicing variants overexpressed in otosclerotic bone. This study is the first comprehensive report to provide evidence for disease associated alternative splicing of CD46.
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References
Karosi T, Szalmás A, Csomor P, Kónya J, Petkó M, Sziklai I (2008) Disease-associated novel CD46 splicing variants and pathologic bone remodeling in otosclerosis. Laryngoscope 118(9):1669-1676
Liszewski MK, Kemper C, Price JD, Atkinson JP (2005) Emerging roles and new functions of CD46. Springer Semin Immunopathol 27(3):345-358
Riley-Vargas RC, Gill DB, Kemper C, Liszewski MK, Atkinson JP (2004) CD46: expanding beyond complement regulation. Trends in Immunol 25(9):496-503
Erlenhofer C, Duprex WP, Rima BK, ter Meulen V, Schneider-Schaulies J (2002) Analysis of receptor (CD46, CD150) usage by measles virus. J Gen Virol 83(6):1431-1436
Dhiman N, Jacobson RM, Poland GA (2004) Measles virus receptors: SLAM and CD46. Rev Med Virol 14(4):217-229
Riley RC, Tannenbaum PL, Abbott DH, Atkinson JP (2002) Cutting edge: inhibiting measles virus infection but promoting reproduction: an explanation for splicing and tissue-specific expression of CD46. J Immunol 169(10):5405-5409
Schneider-Schaulies J, Dunster LM, Kobune F, Rima B, ter Meulen V (1995) Differential downregulation of CD46 by measles virus strains. J Virol 69(11):7257-7259
Manchester M, Naniche D, Stehle T (2000) CD46 as a measles receptor: form follows function. Virology 274(1):5-10
Zaffran Y, Destaing O, Roux A, Ory S, Nheu T, Jurdic P, Rabourdin-Combe C, Astier AL (2001) CD46/CD3 costimulation induces morphological changes of human T cells and activation of Vav, Rac and extracellular signal-regulated kinase mitogen-activated protein kinase. J Immunol 167(12):6780-6785
Kemper C, Chan AC, Green JM, Brett KA, Murphy KM, Atkinson JP (2003) Activation of human CD4+ cells with CD3 and CD46 induces a T-regulatory cell 1 phenotype. Nature 421(6921):388-392
Astier A, Trescol-Biemont MC, Azocar O, Lamouille B, Rabourdin-Combe C (2000) Cutting edge: CD46, a new costimulatory molecular way for T cells that induces p120CBL and LAT phosphorylation. J Immunol 164(12):6091-6095
Kurita-Taniguchi M, Fukui A, Hazeki K, Hirano A, Tsuji S, Matsumoto M, Watanabe M, Ueda S, Seya T (2000) Functional modulation of human macrophages through CD46 (measles virus receptor): production of IL-12 p40 and nitric oxide in association with recruitment of protein-tyrosine phosphatase SHP-1 to CD46. J Immunol 165(9):5143-5152
Acknowledgments
This work was supported by grants from Hungarian Scientific Research Fund (OTKA PD75371) and Mecenatúra Fund of Debrecen University (DE OEC Mec 17/2008).
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Szalmás, A., Kónya, J., Sziklai, I., Karosi, T. (2010). Detection and Identification of CD46 Splicing Isoforms by Nested RT-PCR. In: King, N. (eds) RT-PCR Protocols. Methods in Molecular Biology, vol 630. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-629-0_6
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DOI: https://doi.org/10.1007/978-1-60761-629-0_6
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