Abstract
Extraction of high quality RNA is paramount to successful RT-PCR, and here, a method proven optimal for skeletal muscle is described. While this method described is for use with skeletal muscle, it could be suitable for other types of mammalian tissue also. This method describes an approach to extract high quality RNA with minimal degradation and the subsequent analysis of that RNA in preparation for RT-PCR. Two separate methods of RNA quantification and analysis are described in this chapter and regardless of the method chosen for quantitation and analysis, it is imperative that the integrity of the sample be established before proceeding to RT-PCR.
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Acknowledgments
Thanks to Dr. Robyn M. Murphy and Heidy Latchman for their helpful feedback on the preparation and content of this document.
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Mollica, J.P. (2010). Skeletal Muscle RNA Extraction in Preparation for RT-PCR. In: King, N. (eds) RT-PCR Protocols. Methods in Molecular Biology, vol 630. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-629-0_16
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DOI: https://doi.org/10.1007/978-1-60761-629-0_16
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-628-3
Online ISBN: 978-1-60761-629-0
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