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Recombinant Proteins from Plants

  • Book
  • © 1998

Overview

Editors:
  1. Charles Cunningham

    1. University of Aberdeen, Scotland, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Andrew J. R. Porter

    1. University of Aberdeen, Scotland, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Biotechnology (MIBT, volume 3)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Rice Transformation by Agrobacterium Infection

    • Xiongying Cheng, Ravinder K. Sardana, Illimar Altosaar
    Pages 1-9

  3. Barley as a Producer of Heterologous Protein

    • Leena Mannonen, Kristian Aspegren, Anneli Ritala, Hanna Simola, Teemu H. Teeri
    Pages 11-25

  4. Introducing and Expressing Genes in Legumes

    • Adrian D. Bavage, Mark P. Robbins, Leif Skøt, K. Judith Webb
    Pages 27-38

  5. Use of the GUS Reporter Gene

    • Joy Wilkinson, Keith Lindsey
    Pages 39-47

  6. Expression of Recombinant Proteinase Inhibitors in Plants

    • Dominique Michaud, Thierry C. Vrain
    Pages 49-64

  7. Detection of Recombinant Viral Coat Protein in Transgenic Plants

    • Herta Steinkellner, Irina Korschineck
    Pages 65-75

  8. Synthesis of Recombinant Human Cytokine GM-CSF in the Seeds of Transgenic Tobacco Plants

    • Ravinder K. Sardana, Peter R. Ganz, Anil Dudani, Eilleen S. Tackaberry, Xiongying Cheng, Illimar Altosaar
    Pages 77-87

  9. Tobamovirus Vectors for Expression of Recombinant Genes in Plants

    • Thomas H. Turpen, Stephen J. Reinl
    Pages 89-101

  10. Single-Chain Fv Antibodies Expressed in Plants

    • Udo Conrad, Ulrike Fiedler, Olga Artsaenko, Julian Phillips
    Pages 103-127

  11. Characterization and Applications of Plant-Derived Recombinant Antibodies

    • Rainer Fischer, Jürgen Drossard, Yu-Cai Liao, Stefan Schillberg
    Pages 129-142

  12. Production of Recombinant Antibodies in Plant Suspension Cultures

    • Jürgen Drossard, Yu-Cai Liao, Rainer Fischer
    Pages 143-154

  13. Production of Foreign Proteins in Tobacco Cell Suspension Culture

    • Véronique Gomord, Anne-Catherine Fitchette-Lainé, Lise-Anne Denmat, Dominique Michaud, Loïc Faye
    Pages 155-164

  14. Transient Gene Expression in Plant Protoplasts

    • Stefan Schillberg, Sabine Zimmermann, Dirk Priifer, Detlef Schuman, Rainer Fischer
    Pages 165-175

  15. Stability of Recombinant Proteins in Plants

    • Dominique Michaud, Thierry C. Vrain, Véronique Gomord, Loïc Faye
    Pages 177-188

  16. Trafficking and Stability of Heterologous Proteins in Transgenic Plants

    • Johnathan A. Napier, Gaelle Richard, Peter R. Shewry
    Pages 189-202

  17. Screening for Transgenic Lines with Stable and Suitable Accumulation Levels of a Heterologous Protein

    • Myriam De Neve, Helena Van Houdt, Anne-Marie Bruyns, Marc Van Montagu, Ann Depicker
    Pages 203-227

  18. Manipulation of Photosynthetic Metabolism

    • Martin A. J. Parry, Steven P. Colliver, Pippa J. Madgwick, Matthew J. Paul
    Pages 229-249

  19. Quantification of Heterologous Protein Levels in Transgenic Plants by ELISA

    • Anne-Marie Bruyns, Myriam De Neve, Geert De Jaeger, Chris De Wilde, Pierre Rouzé, Ann Depicker
    Pages 251-269

  20. Analysis of N- and O-Glycosylation of Plant Proteins

    • Anne-Catherine Fitchette-Lainé, Lise-Anne Denmat, Patrice Lerouge, Loïc Faye
    Pages 271-290
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About this book

Recombinant Proteins from Plants is one of the most exciting and fastest developing areas in biology. The latest molecular techniques are being applied to the exploitation of plants as novel expression systems for the p- duction and overproduction of heterologous and native proteins. Transgenic plant technology is currently used in three broad areas: the expression of - combinant proteins to improve crop quality by increasing disease/pest res- tance or increasing tolerance to stress, optimizing plant productivity and yield by the genetic manipulation of metabolic pathways, and the large-scale co- effective production of recombinant proteins for use as specialist industrial or therapeutic biomolecules. The intention of Recombinant Proteins from Plants is to provide c- prehensive and detailed protocols covering all the latest molecular approaches. Because the production oftransgenic plants has become routine in many la- ratories, coverage is also given to some of the more "classical" approaches to the separation, analysis, and characterization of recombinant proteins. The book also includes areas of research that we believe will become increasingly important in the near future: efficient transformation of monocots with Agrobacterium optimizing the stability of recombinant proteins, and a section highlighting the immunotherapeutic potential of plant-expressed proteins.

Editors and Affiliations

  • University of Aberdeen, Scotland, UK

    Charles Cunningham, Andrew J. R. Porter

Bibliographic Information

  • Book Title: Recombinant Proteins from Plants

  • Editors: Charles Cunningham, Andrew J. R. Porter

  • Series Title: Methods in Biotechnology

  • DOI: https://doi.org/10.1007/978-1-60327-260-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1998

  • Hardcover ISBN: 978-0-89603-390-0Published: 24 December 1997

  • Softcover ISBN: 978-1-61737-021-2Published: 19 November 2010

  • eBook ISBN: 978-1-60327-260-5Published: 05 February 2008

  • Series ISSN: 1940-6061

  • Series E-ISSN: 1940-607X

  • Edition Number: 1

  • Number of Pages: XI, 308

  • Topics: Plant Sciences

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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