Abstract
Recent developments in genetic engineering and molecular biology have enabled the production of transgenic plants with improved characteristics, or cell and tissue cultures with altered or improved metabolic activity. However, no universally applicable gene transfer method has hitherto been established for plant transformation. The monocotyledonous plants, including grasses and cereals, have proved to be recalcitrant to genetic engineering. They do not belong to the natural host range of the efficient gene vector Agrobacterium, which is widely used for transformation of dicotyledonous plants. Particle bombardment, developed by Sanford et al. (1), is a physical gene transfer method without target material limitations. Using this method, transgenic plants have been produced successfully from all major cereals (2–8). Protoplast-mediated gene transfer methods, e.g., electroporation or polyethelene glycol (PEG) treatment, have long been hampered by the recalcitrance of important cereal crop species to the regeneration of plants from these protoplasts. However, success has recently been reported in this field, even with elite cultivars of barley (9).
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References
Sanford, J. C., Klein, T. M., Wolff, E. D., and Allen, N. (1987) Delivery of substances into cells and tissues using a particle bombardment process. Particulate Sci. Technol. 5, 3–16.
Gordon-Kamm, W. J., Spencer, T. M., Mangano, M. L., Adams, T. R., Daines, R. J., Start, W. G., O’Brien, J. V., Chambers, S. A., Adams, W. R., Willett, N. G., Rice, T. B., Mackey, C. J., Krueger, R. W., Kausch, A. P., and Lemaux, P. G. (1990) Transformation of maize cells and regeneration of fertile transgenic plants. Plant Cell 2, 603–618.
Christou, P., Ford, T. L., and Kofron, M. (1991) Production of transgenic rice (Oryza sativa L.) plants from agronomically important indica and japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos. Bio/Technology 9, 957–962.
Somers, D. A., Rines, H. W., Weining, G., Kaepler, H. F., and Bushnell, W. R. (1992) Fertile, transgenic oat plants. Bio/Technology 10, 1589–1594.
Vasil, V., Castillo, A. M., Fromm M. E., and Vasil, I. K. (1992) Herbicide resistant transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. Bio/Technology 10, 667–674.
Ritala, A., Aspegren, K., Kurtén, U., Salmenkallio-Marttila, M., Mannonen, L., Hannus, R., Kauppinen, V., Teeri, T. H., and Enari, T. M. (1994) Fertile transgenic barley by particle bombardment if immature embryos. Plant Mol. Biol. 24, 317–325.
Wan, Y. and Lemaux, P. G. (1994) Generation of large number of independently transformed fertile barley plants. Plant Physiol. 104, 37–48.
Castillo, A. M., Vasil, V., and Vasil, I. K. (1994) Rapid production of fertile transgenic plants of rye (Secale cereale L.). Bio/Technology 12, 491–506.
Salmenkallio-Marttila, M., Aspegren, K., Åkerman, S., Kurtén, U., Mannonen, L., Ritala, A., Teeri, T. H., and Kauppinen, V. (1995) Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts. Plant Cell Rep. 15, 301–304.
Aspegren, K., Mannonen, L., Ritala, A., Puupponen-Pimiä, R., Kurtén, U., Salmenkallio-Marttila, M., Kauppinen, V., and Teeri, T. H. (1995) Secretion of a heat-stable fungal β-glucanase from transgenic, suspension-cultured barley cells. Mol. Breeding 1, 91–99.
Mannonen, L. (1993) Barley as a Producer of Heterologous Protein. VTT Publications 138, Espoo, Finland.
Séquin-Schwarz, G., Kott, L., and Kasha, K. J. (1984) Development of haploid cell lines from immature barley Hordeum vulgare embryos. Plant Cell Rep. 3, 95–97.
Ritala, A., Mannonen, L., Salmenkallio-Marttila, M., Kurtén, U., Hannus, R., Aspegren, K., Teeri, T. H., and Kauppinen, V. (1993) Stable transformation of barley tissue culture by particle bombardment. Plant Cell Rep. 12, 435–440.
Dawson, R. M. C., Elliott, D. C., Elliott, W. H., and Jones K. M. (1986) Data for Biochemical Research, 3rd ed., Oxford Science Publications, Clarendon, Oxford, UK.
Sumner, J. B. and Somers G. F. (1949) Dinitrosalisylic method for glucose. Laboratory Experiments in Biological Chemistry. 2nd ed. Academic, New York, pp. 38–39.
Dellaporta, S. L., Wood, J., and Hicks, J. B. (1983) A plant DNA minipreparation∶ version II. Plant. Mol. Biol. Rep. 1, 19–21.
Maniatis, T., Fritsch, E. F., and Shambrook, J. (1982) Molecular Cloning∶ A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
IUPAC (International Union of Pure and Applied Chemistry) (1987) Measurement of cellulase activity. Pure Appl. Chem. 59, 257–268.
Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets∶ procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350–4354.
Niku-Paavola, M.-L. (1991) Isoelectric focusing electrophoresis of lignin. Anal. Biochem. 197, 101–103.
Kvesitadze, E. G., Lomitashvili, T. B., Kvesitadze, G. I., and Niku-Paavola, M.-L. (1992) Thermostable endoglucanases of the thermophilic fungus Allesheria terrestris. Biotechnol. Appl. Biochem. 16, 303–307.
Mannonen, L., Toivonen, L., and Kauppinen, V. (1990) Effects of long-term preservation on growth and productivity of Panax ginseng and Catharanthus roseus cell cultures. Plant Cell Rep. 9, 173–177.
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© 1998 Humana Press Inc., Totowa, NJ
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Mannonen, L., Aspegren, K., Ritala, A., Simola, H., Teeri, T.H. (1998). Barley as a Producer of Heterologous Protein. In: Cunningham, C., Porter, A.J.R. (eds) Recombinant Proteins from Plants. Methods in Biotechnology, vol 3. Humana Press. https://doi.org/10.1007/978-1-60327-260-5_2
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DOI: https://doi.org/10.1007/978-1-60327-260-5_2
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