Overview
- Editors:
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Karina Andrea Gómez
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INGEBI-CONICET, Buenos Aires, Argentina
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Carlos Andrés Buscaglia
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UNSAM-CONICET, IIB-INTECH, San Martin, Argentina
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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About this book
This volume provides detailed descriptions on cutting-edge experimental methods to assess the multiple and complex interactions between T. cruzi and its host. Chapters detail a series of methods and protocols, ranging from rather classical biochemical/genetic approaches to modern, powerful and high-throughput ‘-omics’ technologies, to tackle this issue, as well as novel techniques to improve treatment and clinical evaluation of Chagasic patients. Genome-wide mining strategies aimed at identifying potential parasite antigens and vaccine candidates and an overview on the challenges and milestones encountered during T. cruzi genome assembly are also included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, T. cruzi Infection: Methods and Protocols aims to provide students, researchers, and clinicians who are interested in the study of Chagas disease an indispensable source of information.
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Article
Open access
21 August 2017
Table of contents (28 protocols)
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- Elizabeth Ruiz-Lancheros, Makan Golizeh, Momar Ndao
Pages 263-273
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- Julio Alonso-Padilla, Dolors Tassies, Nuria Cortes-Serra, Joaquim Gascon, Joan-Carles Reverter, María-Jesús Pinazo
Pages 275-286
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- Uriel Ortega-Rodriguez, Susana Portillo, Roger A. Ashmus, Jerry A. Duran, Nathaniel S. Schocker, Eva Iniguez et al.
Pages 287-308
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- Nayara I. Medeiros, Juliana A. S. Gomes
Pages 309-314
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- Gonzalo R. Acevedo, Paula B. Alcaráz, Clemencia Pinilla, Karina A. Gómez
Pages 315-337
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- María Cecilia Albareda, María Ailén Natale, Gonzalo Leandro Cesar, Melisa Daiana Castro Eiro, María Gabriela Alvarez, Susana Adriana Laucella
Pages 339-348
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- Paola Lasso, Jose Mateus, John Mario González, Adriana Cuéllar, Concepción Puerta
Pages 349-361
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- John E. Wiktorowicz, M. Paola Zago, Nisha J. Garg
Pages 363-380
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- Natalia Eberhardt, Liliana María Sanmarco, Maria Pilar Aoki
Pages 381-395
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Back Matter
Pages 397-398
Reviews
“The book is written for students, active researchers, and clinicians. … This book comprehensively covers the experimental methods for the study of the biology of T. cruzi and its complex interactions with the human host. The unique feature of the book is that the variety of methods and protocols it covers. There are very few books that provide the depth of coverage this book does.” (Omer Iqbal, Doody's Book Reviews, August 2019)
Editors and Affiliations
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INGEBI-CONICET, Buenos Aires, Argentina
Karina Andrea Gómez
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UNSAM-CONICET, IIB-INTECH, San Martin, Argentina
Carlos Andrés Buscaglia