Abstract
Cytometric bead array (CBA) is a flow cytometry application that allows users to quantify multiple proteins simultaneously. Compared to other quantifier assays, as enzyme-linked immunosorbent assay (ELISA) and Western blot, CBA significantly reduces sample requirements and time to results. This technology allows for the design and creation of assays to measure a variety of analytes including inflammatory mediators, chemokines, immunoglobulin isotypes, intracellular signaling molecules, apoptotic mediators, adhesion molecules, and antibodies. Here we describe CBA steps to measure soluble cytokine levels in body fluids of infected patients by the protozoan Trypanosoma cruzi, morbidity known as Chagas disease.
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References
Zhang JM, An J (2007) Cytokines, inflammation, and pain. Int Anesthesiol Clin 45(2):27–37. https://doi.org/10.1097/AIA.0b013e318034194e
Burska A, Boissinot M, Ponchel F (2014) Cytokines as biomarkers in rheumatoid arthritis. Mediat Inflamm 2014:545493. https://doi.org/10.1155/2014/545493
Cassirer-Costa F, Medeiros NI, Chaves AT, Lyon S, Coelho-Dos-Reis JGA, Ribeiro-Junior AF, Correa-Oliveira R, Rocha MOC, Gomes JAS (2017) Cytokines as biomarkers to monitoring the impact of multidrug therapy in immune response of leprosy patients. Cytokine 97:42–48. https://doi.org/10.1016/j.cyto.2017.05.020
Sugama K, Suzuki K, Yoshitani K, Shiraishi K, Kometani T (2013) Urinary excretion of cytokines versus their plasma levels after endurance exercise. Exerc Immunol Rev 19:29–48
Enriquez-de-Salamanca A, Castellanos E, Stern ME, Fernandez I, Carreno E, Garcia-Vazquez C, Herreras JM, Calonge M (2010) Tear cytokine and chemokine analysis and clinical correlations in evaporative-type dry eye disease. Mol Vis 16:862–873
Riis JL, Granger DA, DiPietro JA, Bandeen-Roche K, Johnson SB (2015) Salivary cytokines as a minimally-invasive measure of immune functioning in young children: correlates of individual differences and sensitivity to laboratory stress. Dev Psychobiol 57(2):153–167. https://doi.org/10.1002/dev.21271
Sousa GR, Gomes JA, Fares RC, Damasio MP, Chaves AT, Ferreira KS, Nunes MC, Medeiros NI, Valente VA, Correa-Oliveira R, Rocha MO (2014) Plasma cytokine expression is associated with cardiac morbidity in Chagas disease. PLoS One 9(3):e87082. https://doi.org/10.1371/journal.pone.0087082
Volta BJ, Bustos PL, Cardoni RL, De Rissio AM, Laucella SA, Bua J (2016) Serum cytokines as biomarkers of early Trypanosoma cruzi infection by congenital exposure. J Immunol 196(11):4596–4602. https://doi.org/10.4049/jimmunol.1502504
Sousa GR, Gomes JA, Damasio MP, Nunes MC, Costa HS, Medeiros NI, Fares RC, Chaves AT, Correa-Oliveira R, Rocha MO (2017) The role of interleukin 17-mediated immune response in Chagas disease: high level is correlated with better left ventricular function. PLoS One 12(3):e0172833. https://doi.org/10.1371/journal.pone.0172833
Fares RC, Gomes Jde A, Garzoni LR, Waghabi MC, Saraiva RM, Medeiros NI, Oliveira-Prado R, Sangenis LH, Chambela Mda C, de Araujo FF, Teixeira-Carvalho A, Damasio MP, Valente VA, Ferreira KS, Sousa GR, Rocha MO, Correa-Oliveira R (2013) Matrix metalloproteinases 2 and 9 are differentially expressed in patients with indeterminate and cardiac clinical forms of Chagas disease. Infect Immun 81(10):3600–3608. https://doi.org/10.1128/IAI.00153-13
Sousa GR, Gomes JAS, Fares RCG, Damasio MPS, Chaves AT, Ferreira KS, Medeiros NI, Nunes MCP, Correa-Oliveira R, Rocha MOC (2013) A new highly sensible method for division of patients with different forms of Chagas disease according to cytokine production. Front Immunol. https://doi.org/10.3389/conf.fimmu.2013.02.01191
Keustermans GC, Hoeks SB, Meerding JM, Prakken BJ, de Jager W (2013) Cytokine assays: an assessment of the preparation and treatment of blood and tissue samples. Methods 61(1):10–17. https://doi.org/10.1016/j.ymeth.2013.04.005
Morgan E, Varro R, Sepulveda H, Ember JA, Apgar J, Wilson J, Lowe L, Chen R, Shivraj L, Agadir A, Campos R, Ernst D, Gaur A (2004) Cytometric bead array: a multiplexed assay platform with applications in various areas of biology. Clin Immunol 110(3):252–266. https://doi.org/10.1016/j.clim.2003.11.017
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Medeiros, N.I., Gomes, J.A.S. (2019). Cytometric Bead Array (CBA) for Measuring Cytokine Levels in Chagas Disease Patients. In: Gómez, K., Buscaglia, C. (eds) T. cruzi Infection. Methods in Molecular Biology, vol 1955. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9148-8_23
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DOI: https://doi.org/10.1007/978-1-4939-9148-8_23
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