Immune Checkpoint Blockade

Methods and Protocols

  • Yago Pico de Coaña

Part of the Methods in Molecular Biology book series (MIMB, volume 1913)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Generating Hypothesis

    1. Front Matter
      Pages 1-1
    2. Pablo Moreno-Ruiz, Lina Wik Leiss, Artur Mezheyeuski, Monika Ehnman
      Pages 3-11
    3. Claudia Wickenhauser, Daniel Bethmann, Zipei Feng, Shawn M. Jensen, Carmen Ballesteros-Merino, Chiara Massa et al.
      Pages 13-31
    4. Tadepally Lakshmikanth, Petter Brodin
      Pages 33-48
    5. Shi Yong Neo, Aine O’Reilly, Yago Pico de Coaña
      Pages 49-65
    6. Fabio Marino, Chloe Chong, Justine Michaux, Michal Bassani-Sternberg
      Pages 67-79
    7. Uta Sandy Tretbar, Michael Friedrich, Maria-Filothei Lazaridou, Barbara Seliger
      Pages 81-101
  3. Functional Assays

    1. Front Matter
      Pages 103-103
    2. Stina Wickström, Tanja Lövgren
      Pages 105-118
    3. Nataly Cruz Rodríguez, Johanna Lineros, Carol Stefany Rodríguez, Lina María Martínez, Josefa Antonia Rodríguez
      Pages 119-131
    4. Alexander Martens, Graham Pawelec, Christopher Shipp
      Pages 141-151
    5. Irene van der Haar Àvila, Patricia Marmol, Rolf Kiessling, Yago Pico de Coaña
      Pages 167-179
    6. Irene van der Haar Àvila, Patricia Marmol, Jeannette Cany, Rolf Kiessling, Yago Pico de Coaña
      Pages 181-194
  4. Animal Models

    1. Front Matter
      Pages 195-195
    2. Felipe Galvez-Cancino, Ernesto Lopez, Alvaro Lladser
      Pages 217-222
  5. Back Matter
    Pages 223-225

About this book


This detailed volume describes a series of techniques that are essential for evaluating the efficacy of new checkpoint blockade therapies as well as understanding the mechanisms behind the therapies that have already been approved. Beginning with a section on describing the tumor microenvironment and evaluating the immune system at a systemic level, the book continues by covering functional assays that provide answers to questions that may be raised after studying the immune system and its responses to immunotherapies, as well as the use of animal models in this research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Immune Checkpoint Blockade: Methods and Protocols serves to aid researchers in furthering our understanding of checkpoint blockage as well as the study of tumor immunology and the development of new immunotherapies.


Tumor immunology Immunotherapy Antitumoral immune responses Functional assays Cancer cell lines Translational research Checkpoint blockade antibody

Editors and affiliations

  • Yago Pico de Coaña
    • 1
  1. 1.Department of Oncology-PathologyKarolinska InstitutetStockholmSweden

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8978-2
  • Online ISBN 978-1-4939-8979-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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