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Immune Monitoring of Cancer Patients by Multi-color Flow Cytometry

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Immune Checkpoint Blockade

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1913))

Abstract

The irruption of immune-activating therapies to treat cancer has created a need for evaluating both the response and possible adverse events related to these novel treatments. Multicolor flow cytometry is a powerful tool that enables tumor immunologists to characterize the immune system of patients before and in response to immunotherapy. We present here a protocol for purifying human peripheral blood mononuclear cells and staining them with a set of six multicolor panels that allow for a thorough characterization of the immune system of healthy donors as well as patients that are undergoing treatments that may modify the immune system.

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Authors and Affiliations

  1. Department of Oncology-Pathology, Karolinska Institute, Stockholm, Sweden

    Shi Yong Neo, Aine O’Reilly & Yago Pico de Coaña

Authors
  1. Shi Yong Neo
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  2. Aine O’Reilly
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  3. Yago Pico de Coaña
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Editors and Affiliations

  1. Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden

    Yago Pico de Coaña

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Neo, S.Y., O’Reilly, A., Pico de Coaña, Y. (2019). Immune Monitoring of Cancer Patients by Multi-color Flow Cytometry. In: Pico de Coaña, Y. (eds) Immune Checkpoint Blockade. Methods in Molecular Biology, vol 1913. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8979-9_4

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  • DOI: https://doi.org/10.1007/978-1-4939-8979-9_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8978-2

  • Online ISBN: 978-1-4939-8979-9

  • eBook Packages: Springer Protocols

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