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Fast Detection of DNA Damage

Methods and Protocols

  • Vladimir V. Didenko

Part of the Methods in Molecular Biology book series (MIMB, volume 1644)

Table of contents

  1. Front Matter
    Pages i-x
  2. Express Detection (from 3 min to 1 h)

    1. Front Matter
      Pages 1-1
    2. Hadi Tadayyon, Mehrdad J. Gangeh, Roxana Vlad, Michael C. Kolios, Gregory J. Czarnota
      Pages 23-40
    3. Hadi Tadayyon, Mehrdad J. Gangeh, Roxana Vlad, Michael C. Kolios, Gregory J. Czarnota
      Pages 41-60
    4. Zbigniew Darzynkiewicz, Hong Zhao, H. Dorota Halicka, Piotr Pozarowski, Brian Lee
      Pages 61-73
    5. Piero Sestili, Cinzia Calcabrini, Anna Rita Diaz, Carmela Fimognari, Vilberto Stocchi
      Pages 75-93
    6. Fátima Otero, Rebeca Santiso, María Tamayo, Germán Bou, Jaime Gosálvez, José Luis Fernández
      Pages 95-104
    7. Igor Kudryavtsev, Maria Serebryakova, Liudmila Solovjeva, Maria Svetlova, Denis Firsanov
      Pages 105-111
    8. Candace L. Minchew, Vladimir V. Didenko
      Pages 113-119
  3. Rapid Detection (2–3 h)

    1. Front Matter
      Pages 121-121
    2. Nate Hopp, Jodi Hagen, Birte Aggeler, Alexander E. Kalyuzhny
      Pages 123-128
    3. Denis Firsanov, Liudmila Solovjeva, Olga Lublinskaya, Valeriy Zenin, Igor Kudryavtsev, Maria Serebryakova et al.
      Pages 129-138
  4. Accelerated Detection (Time-Saving Versions of Conventional Techniques)

    1. Front Matter
      Pages 179-179
    2. Sergey Shaposhnikov, Andrew Collins
      Pages 181-186
    3. Liudmila Solovjeva, Denis Firsanov, Nadezhda Pleskach, Maria Svetlova
      Pages 187-194
  5. Back Matter
    Pages 213-216

About this book

Introduction

This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the “research trenches” they are not covered well in the literature. This volume is the first such compendium of the time-saving techniques for detection of DNA damage and its direct physiological outcomes including apoptosis, necrosis and phagocytic clearance. The volume demonstrates all levels of detection, starting from the molecular level up to the level of the entire live organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Fast Detection of DNA Damage: Methods and Protocols aims to provide easily reproducible techniques requiring only few steps to perform.

Keywords

FRET reporting DNA breaks mitochondrial DNA damage bacterial genomes invertebrate species genotoxin-induced DNA damage γ-H2AX foci

Editors and affiliations

  • Vladimir V. Didenko
    • 1
  1. 1.Department of Neurosurgery and Department of Molecular and Cellular BiologyBaylor College of MedicineHoustonUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7187-9
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7185-5
  • Online ISBN 978-1-4939-7187-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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