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Ultrasound Imaging of DNA-Damage Effects in Live Cultured Cells and in Brain Tissue

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Fast Detection of DNA Damage

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1644))

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Abstract

High-frequency ultrasound (>20 MHz) spectroscopy can be used to detect noninvasively DNA damage in cell samples in vitro, and in live tissue both ex vivo and in vivo. This chapter focuses on the former two aspects. Experimental evidence suggests that morphological changes that occur in cells undergoing apoptosis result in changes in frequency-dependent ultrasound backscatter. With advances in research, ultrasound spectroscopy is advancing the boundaries of fast, label-free, noninvasive DNA damage detection technology with potential use in personalized medicine and early therapy response monitoring. Depending on the desired resolution, parametric ultrasound images can be computed and displayed within minutes to hours after ultrasound examination for cell death.

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Acknowledgments

M.J.G. holds the Natural Sciences and Engineering Research Council of Canada Post-doctoral Fellowship. G.J.C. holds a University of Toronto James and Mary Davie Chair in Breast Cancer Imaging and Ablation. Funding for these projects was provided by the Terry Fox Foundation.

Author information

Authors and Affiliations

  1. Physical Sciences, Sunnybrook Research Institute, Sunnybrook Health Sciences Centre, 2075 Bayview Avenue T2, Toronto, ON, Canada, M4N 3M5

    Hadi Tadayyon, Mehrdad J. Gangeh & Gregory J. Czarnota Ph.D., M.D.

  2. Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada, M4N 3M5

    Hadi Tadayyon, Mehrdad J. Gangeh & Gregory J. Czarnota Ph.D., M.D.

  3. Department of Radiation Oncology, Sunnybrook Health Sciences Centre, Toronto, ON, Canada, M4N 3M5

    Hadi Tadayyon, Mehrdad J. Gangeh & Gregory J. Czarnota Ph.D., M.D.

  4. Department of Radiation Oncology, Juravinski Cancer Centre, Hamilton Health Sciences, Hamilton, ON, Canada, L8V 5C2

    Roxana Vlad

  5. Department of Physics, Ryerson University, Toronto, ON, Canada, M5B 2K3

    Michael C. Kolios

  6. Department of Radiation Oncology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada, M5G 2M9

    Gregory J. Czarnota Ph.D., M.D.

Authors
  1. Hadi Tadayyon
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  2. Mehrdad J. Gangeh
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  3. Roxana Vlad
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  4. Michael C. Kolios
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  5. Gregory J. Czarnota Ph.D., M.D.
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Corresponding author

Correspondence to Gregory J. Czarnota Ph.D., M.D. .

Editor information

Editors and Affiliations

  1. Department of Neurosurgery and Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA

    Vladimir V. Didenko

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Tadayyon, H., Gangeh, M.J., Vlad, R., Kolios, M.C., Czarnota, G.J. (2017). Ultrasound Imaging of DNA-Damage Effects in Live Cultured Cells and in Brain Tissue. In: Didenko, V. (eds) Fast Detection of DNA Damage. Methods in Molecular Biology, vol 1644. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7187-9_3

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  • DOI: https://doi.org/10.1007/978-1-4939-7187-9_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7185-5

  • Online ISBN: 978-1-4939-7187-9

  • eBook Packages: Springer Protocols

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