Bacterial Cell Wall Homeostasis

Methods and Protocols

  • Hee-Jeon Hong

Part of the Methods in Molecular Biology book series (MIMB, volume 1440)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Analyzing Physical Properties of the Bacterial Cell Wall

    1. Front Matter
      Pages 1-1
    2. Robert D. Turner, Jamie K. Hobbs, Simon J. Foster
      Pages 3-9
  3. Genome-Wide Approaches for the Identification of Gene Products with Roles in Cell Wall Homeostasis

    1. Front Matter
      Pages 29-29
    2. Hee-Jeon Hong, Andy Hesketh
      Pages 31-46
    3. Alessandra M. Martorana, Sara Motta, Paola Sperandeo, Pierluigi Mauri, Alessandra Polissi
      Pages 57-70
    4. Ruth Reyes-Cortés, Emma S. Arguijo-Hernández, Marco A. Carballo-Ontiveros, Eva Martínez-Peñafiel, Luis Kameyama
      Pages 71-83
  4. Functional Analysis of Cell-Wall Associated Proteins

    1. Front Matter
      Pages 85-85
    2. Tatsuya Fukushima, Junichi Sekiguchi
      Pages 87-98
    3. Andrew Duong, Kalinka Koteva, Danielle L. Sexton, Marie A. Elliot
      Pages 99-108
    4. Chenggang Wu, Melissa Elizabeth Reardon-Robinson, Hung Ton-That
      Pages 109-122
  5. Reporter Assays for Cell Wall Stress

  6. Analysis of the Non-Protein Components of the Cell Wall

    1. Front Matter
      Pages 151-151
    2. Elisa Binda, Lùcia Carrano, Giorgia Letizia Marcone, Flavia Marinelli
      Pages 153-170
    3. Alexander J. F. Egan, Waldemar Vollmer
      Pages 171-184
    4. Ryan E. Schaub, Jonathan D. Lenz, Joseph P. Dillard
      Pages 185-200

About this book

Introduction

This volume brings together the most widely used and important protocols currently being employed in researching and understanding bacterial cell wall homeostasis. Chapters in Bacterial Cell Wall Homeostasis cover a variety of subjects, such as: modern microscopy techniques and other biophysical methods used to characterize the subcellular structure of the bacterial cell wall; high-throughput approaches that can be used to identify all the genes and proteins that participate in the correct functioning of an organism’s cell wall; protocols for assaying individual gene products for specific cell wall functions or identify chemicals with inhibitory activity against the cell wall; and methods for analyzing the non-protein components of the cell wall and the increasing use of computational approaches for predicting and modeling cell wall related functions and processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Bacterial Cell Wall Homeostasis: Methods and Protocols emphasizes the diversity of the research taking place in bacterial cell wall homeostasis, and explains how the integration of information from across multiple disciplines is going to be essential if a holistic understanding of this important process is to be obtained.

 

Keywords

single celled organism subcellular structure high-throughput approaches gene expression non-protein components of the cell wall peptidoglycan

Editors and affiliations

  • Hee-Jeon Hong
    • 1
  1. 1.Dept. of Biochem & Dept. of Bio and Med.Univ. of Cambridge andOxford Brookes UnvCambridgeUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-3676-2
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3674-8
  • Online ISBN 978-1-4939-3676-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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