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Gastrointestinal Physiology and Diseases

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Andrei I. Ivanov

    1. Dept of Human and Molecular Genetics, VCU Institute of Molecular Medicine, VCU, Richmond, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1422)

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Table of contents (29 protocols)

  1. Isolation and Characterization of Intestinal Immune Cells

    1. Isolation and Functional Analysis of Lamina Propria Dendritic Cells from the Mouse Small Intestine

      • Naoki Takemura, Satoshi Uematsu
      Pages 181-188

    2. Purification and Adoptive Transfer of Group 3 Gut Innate Lymphoid Cells

      • Xiaohuan Guo, Kevin Muite, Joanna Wroblewska, Yang-Xin Fu
      Pages 189-196

    3. Immunotherapy with iTreg and nTreg Cells in a Murine Model of Inflammatory Bowel Disease

      • Dipica Haribhai, Talal A. Chatila, Calvin B. Williams
      Pages 197-211

    4. Isolation of Eosinophils from the Lamina Propria of the Murine Small Intestine

      • Claudia Berek, Alexander Beller, Van Trung Chu
      Pages 213-221

  2. Animal Models of Gastrointestinal Inflammation and Injury

    1. Front Matter

      Pages 223-223
      Download chapter PDF

    2. Investigation of Host and Pathogen Contributions to Infectious Colitis Using the Citrobacter rodentium Mouse Model of Infection

      • Else S. Bosman, Justin M. Chan, Kirandeep Bhullar, Bruce A. Vallance
      Pages 225-241

    3. Murine Trinitrobenzoic Acid-Induced Colitis as a Model of Crohn’s Disease

      • John F. Kuemmerle
      Pages 243-252

    4. Oxazolone-Induced Colitis as a Model of Th2 Immune Responses in the Intestinal Mucosa

      • Benno Weigmann, Markus F. Neurath
      Pages 253-261

    5. The Mongolian Gerbil: A Robust Model of Helicobacter pylori-Induced Gastric Inflammation and Cancer

      • Jennifer M. Noto, Judith Romero-Gallo, M. Blanca Piazuelo, Richard M. Peek
      Pages 263-280

    6. A Rapid Screenable Assay for Compounds That Protect Against Intestinal Injury in Zebrafish Larva

      • Jason R. Goldsmith, Sarah Tomkovich, Christian Jobin
      Pages 281-293

  3. Animal Models of Gastrointestinal Cancer

    1. Front Matter

      Pages 295-295
      Download chapter PDF

    2. AOM/DSS Model of Colitis-Associated Cancer

      • Bobak Parang, Caitlyn W. Barrett, Christopher S. Williams
      Pages 297-307

    3. Characterization of Colorectal Cancer Development in Apc min/+ Mice

      • ILKe Nalbantoglu, Valerie Blanc, Nicholas O. Davidson
      Pages 309-327

    4. Modeling Murine Gastric Metaplasia Through Tamoxifen-Induced Acute Parietal Cell Loss

      • Jose B. Saenz, Joseph Burclaff, Jason C. Mills
      Pages 329-339

    5. The Hamster Buccal Pouch Model of Oral Carcinogenesis

      • Siddavaram Nagini, Jaganathan Kowshik
      Pages 341-350

  4. Back Matter

    Pages 351-353
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Keywords

About this book

This volume provides a comprehensive collection of  classical and cutting edge protocols and techniques to examine the normal development and physiological functions of the gastrointestinal system and to model the most common digestive diseases. The chapters focus on diverse research topics including ex vivo systems to study gastrointestinal development and functions, in vivo imaging of the gastrointestinal tract, isolation and characterization of intestinal immune cells, and animal models of gastrointestinal inflammation and cancer. The Gastrointestinal Physiology and Diseases: Methods and Protocols book targets wide audience of physiologists, cell and developmental biologists, immunologists, and physician-scientists working in the field of gastroenterology and beyond. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Highly practical and clearly written, Gastrointestinal Physiology and Diseases: Methods and Protocols will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining normal functions and diseases of the gastrointestinal tract.


Editors and Affiliations

  • Dept of Human and Molecular Genetics, VCU Institute of Molecular Medicine, VCU, Richmond, USA

    Andrei I. Ivanov

Bibliographic Information

  • Book Title: Gastrointestinal Physiology and Diseases

  • Book Subtitle: Methods and Protocols

  • Editors: Andrei I. Ivanov

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3603-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3601-4Published: 01 June 2016

  • Softcover ISBN: 978-1-4939-8091-8Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3603-8Published: 31 May 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 353

  • Number of Illustrations: 25 b/w illustrations, 62 illustrations in colour

  • Topics: Molecular Medicine

  • Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Health & Hospitals, Pharma

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