Abstract
Only recently has it become apparent that eosinophils play a crucial role in mucosal immune homeostasis. Although eosinophils are the main cellular component of the lamina propria of the gastrointestinal tract, they have often been overlooked because they express numerous markers, which are normally used to characterize macrophages and/or dendritic cells. To study their function in mucosal immunity, it is important to isolate them with high purity and viability. Here, we describe a protocol to purify eosinophils from the lamina propria of the murine small intestine. The method involves preparation of the small intestine, removal of epithelial cells and digestion of the lamina propria to release eosinophils. A protocol to sort eosinophils is included.
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Acknowledgement
The work was supported by the DFG grant (BE 1171/2). The DRFZ, an Institute of the Leibniz Association, is supported by the Berlin Senate of Research and Education.
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Berek, C., Beller, A., Chu, V.T. (2016). Isolation of Eosinophils from the Lamina Propria of the Murine Small Intestine. In: Ivanov, A. (eds) Gastrointestinal Physiology and Diseases. Methods in Molecular Biology, vol 1422. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3603-8_20
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DOI: https://doi.org/10.1007/978-1-4939-3603-8_20
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3601-4
Online ISBN: 978-1-4939-3603-8
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