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Photoswitching Proteins

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Sidney Cambridge

    1. Institute of Anatomy and Cell Biology, University of Heidelberg, Heidelberg, Germany

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  • Presents current up-to-date methodologies
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1148)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Photoinduced Damage Resulting from Fluorescence Imaging of Live Cells

    • Matthew K. Daddysman, Michael A. Tycon, Christopher J. Fecko
    Pages 1-17

  3. Modification of Purified Proteins with Photochemical Protection Compounds for High-Resolution Photoactivation of Protein Function In Vitro and In Vivo

    • Sidney Cambridge
    Pages 19-30

  4. Optochemical Activation of Kinase Function in Live Cells

    • Andrei V. Karginov, Klaus M. Hahn, Alexander Deiters
    Pages 31-43

  5. Photoswitching of Cell Surface Receptors Using Tethered Ligands

    • Andreas Reiner, Ehud Y. Isacoff
    Pages 45-68

  6. Photocontrol of AMPA Receptors with a Photochromic Ligand

    • Martin Sumser, Philipp Stawski
    Pages 69-76

  7. Photoconversion of CFP to Study Neuronal Tissue with Electron Microscopy

    • Nina Wittenmayer
    Pages 77-87

  8. Light-Inducible Gene Regulation with Engineered Zinc Finger Proteins

    • Lauren R. Polstein, Charles A. Gersbach
    Pages 89-107

  9. Manipulation of Plasma Membrane Phosphoinositides Using Photoinduced Protein–Protein Interactions

    • Olof Idevall-Hagren, Pietro De Camilli
    Pages 109-128

  10. A Roadmap to Applying Optogenetics in Neuroscience

    • Consuelo Fois, Pierre-Hugues Prouvot, Albrecht Stroh
    Pages 129-147

  11. Salvaging Ruins: Reverting Blind Retinas into Functional Visual Sensors

    • Marion Mutter, Natalia Swietek, Thomas A. Münch
    Pages 149-160

  12. Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal Activity

    • Wagner Steuer Costa, Jana Liewald, Alexander Gottschalk
    Pages 161-175

  13. Structural Basis of Photoswitching in Fluorescent Proteins

    • Chenxi Duan, Virgile Adam, Martin Byrdin, Dominique Bourgeois
    Pages 177-202

  14. Using Photoactivatable GFP to Track Axonal Transport Kinetics

    • Archan Ganguly, Subhojit Roy
    Pages 203-215

  15. In Vivo Cell Tracking Using PhOTO Zebrafish

    • William P. Dempsey, Hanyu Qin, Periklis Pantazis
    Pages 217-228

  16. In Vivo Optogenetics for Light-Induced Oxidative Stress in Transgenic Zebrafish Expressing the KillerRed Photosensitizer Protein

    • Cathleen Teh, Vladimir Korzh
    Pages 229-238

  17. Photoactivatable Fluorescent Proteins for Super-resolution Microscopy

    • Yuji Ishitsuka, Karin Nienhaus, G. Ulrich Nienhaus
    Pages 239-260

  18. pcSOFI as a Smart Label-Based Superresolution Microscopy Technique

    • Benjamien Moeyaert, Peter Dedecker
    Pages 261-276

  19. Back Matter

    Pages 277-278
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Keywords

About this book

Photoswitching Proteins: Methods and Protocols, focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Photoswitching Proteins: Methods and Protocols highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems.

Editors and Affiliations

  • Institute of Anatomy and Cell Biology, University of Heidelberg, Heidelberg, Germany

    Sidney Cambridge

Bibliographic Information

  • Book Title: Photoswitching Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Sidney Cambridge

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0470-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-4939-0469-3Published: 10 April 2014

  • Softcover ISBN: 978-1-4939-5435-3Published: 17 September 2016

  • eBook ISBN: 978-1-4939-0470-9Published: 09 April 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 278

  • Number of Illustrations: 53 b/w illustrations, 5 illustrations in colour

  • Topics: Protein Science, Biological Microscopy, Neurobiology

  • Industry Sectors: Biotechnology, Pharma

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