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Circulating Nucleic Acids in Serum and Plasma – CNAPS IX

  • Conference proceedings
  • © 2016

Overview

Editors:
  1. Peter B. Gahan

    1. ICRM, Inserm, Montpellier Cedex, France

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  2. Michael Fleischhacker

    1. Universitätsklinikum Halle (Saale), Klinik für Innere Medizin, Schwerpunkt Pneumologie, Halle/Saale, Germany

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  3. Bernd Schmidt

    1. Universitätsklinikum Halle (Saale), Klinik für Innere Medizin, Schwerpunkt Pneumologie, Halle/Saale, Germany

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  • Proceedings of the IX International Meeting on Circulating Nucleic Acids in Plasma and Serum (CNAPS)
  • The latest research in the field of circulating nucleic acids
  • An overview of its application to: basic and applied cancer research; the analysis of circulating fetal DNA (i.e. clinical tests for the detection of foetuses with chromosome aberrations); and the biological basis of extracellular nucleic acids

Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 924)

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Table of contents (36 papers)

  1. Biology of CNAPS

    1. A Historical and Evolutionary Perspective on Circulating Nucleic Acids and Extracellular Vesicles: Circulating Nucleic Acids as Homeostatic Genetic Entities

      • Janine Aucamp, Abel Jacobus Bronkhorst, Piet J. Pretorius
      Pages 91-95

    2. Comparison of MicroRNA Content in Plasma and Urine Indicates the Existence of a Transrenal Passage of Selected MicroRNAs

      • Eva Pazourkova, Sarka Pospisilova, Iveta Svobodova, Ales Horinek, Antonin Brisuda, Viktor Soukup et al.
      Pages 97-100

    3. A Quantitative Assessment of Cell-Free DNA Utilizing Several Housekeeping Genes: Measurements from Four Different Cell Lines

      • Janine Aucamp, Abel Jacobus Bronkhorst, Johannes F. Wentzel, Piet J. Pretorius
      Pages 101-103

    4. Oligodeoxynucleotide Analogues of Circulating DNA Inhibit dsRNA-Induced Immune Response at the Early Stages of Signal Transduction Cascade in a Cell Type-Dependent Manner

      • Anna V. Cherepanova, Zhanna K. Nazarkina, Pavel P. Laktionov
      Pages 105-107

    5. GC-Rich DNA Fragments and Oxidized Cell-Free DNA Have Different Effects on NF-kB and NRF2 Signaling in MSC

      • Vasilina A. Sergeeva, Svetlana V. Kostyuk, Elizaveta S. Ershova, Elena M. Malinovskaya, Tatiana D. Smirnova, Larisa V. Kameneva et al.
      Pages 109-112

    6. Evaluation of the State of Transplanted Liver Health by Monitoring of Organ-Specific Genomic Marker in Circulating DNA from Receptor

      • Hada C. Macher, G. Suárez-Artacho, Pilar Jiménez-Arriscado, S. Álvarez-Gómez, N. García-Fernández, Juan M. Guerrero et al.
      Pages 113-116

    7. Vesicular and Extra-Vesicular RNAs of Human Blood Plasma

      • Anna V. Savelyeva, Dmitriy N. Baryakin, Elena D. Chikova, Elena V. Kuligina, Vladimir A. Richter, Dmitriy V. Semenov
      Pages 117-119

    8. Artificial Analogues of Circulating Box C/D RNAs Induce Strong Innate Immune Response and MicroRNA Activation in Human Adenocarcinoma Cells

      • Grigory A. Stepanov, Julia A. Filippova, Anna A. Nushtaeva, Elena V. Kuligina, Olga A. Koval, Vladimir A. Richter et al.
      Pages 121-125

    9. Multiple Ways of cfDNA Reception and Following ROS Production in Endothelial Cells

      • Anna Yu. Alekseeva, Larisa V. Kameneva, Svetlana V. Kostyuk, Natalia N. Veiko
      Pages 127-131

    10. Protein Content of Circulating Nucleoprotein Complexes

      • Svetlana N. Tamkovich, Oleg S. Tutanov, Danil S. Serdukov, Maxim S. Belenikin, Anatoliy G. Shlikht, Natalia A. Kirushina et al.
      Pages 133-136

  2. Methodology

    1. Front Matter

      Pages 137-137
      Download chapter PDF

    2. Digital PCR of Genomic Rearrangements for Monitoring Circulating Tumour DNA

      • Hongdo Do, Daniel Cameron, Ramyar Molania, Bibhusal Thapa, Gareth Rivalland, Paul L. Mitchell et al.
      Pages 139-146

    3. mFast-SeqS as a Monitoring and Pre-screening Tool for Tumor-Specific Aneuploidy in Plasma DNA

      • Jelena Belic, Marina Koch, Peter Ulz, Martina Auer, Teresa Gerhalter, Sumitra Mohan et al.
      Pages 147-155

    4. Methodological Variables in the Analysis of Cell-Free DNA

      • Abel Jacobus Bronkhorst, Janine Aucamp, Piet J. Pretorius
      Pages 157-163

    5. Novel Technology for Enrichment of Biomolecules from Cell-Free Body Fluids and Subsequent DNA Sizing

      • Vipulkumar Patel, Peter Celec, Magdalena Grunt, Heidi Schwarzenbach, Ingo Jenneckens, Timo Hillebrand
      Pages 165-169

    6. A Rapid and Sensitive Method for Detection of the T790M Mutation of EGFR in Plasma DNA

      • Hideharu Kimura, Shingo Nishikawa, Hayato Koba, Taro Yoneda, Takashi Sone, Kazuo Kasahara
      Pages 171-174

    7. Evaluation of Different Blood Collection Tubes and Blood Storage Conditions for the Preservation and Stability of Cell-Free Circulating DNA for the Analysis of the Methylated mSEPT9 Colorectal Cancer Screening Marker

      • Jurgen Distler, Reimo Tetzner, Gunter Weiss, Thomas König, Anne Schlegel, Michal Bagrowski
      Pages 175-178

    8. Purification of Circulating Cell-Free DNA from Plasma and Urine Using the Automated Large-Volume Extraction on the QIAsymphony® SP Instrument

      • Alexander Wolf, Katharina Beller, Sebastian Groemminger, Wera Hofmann, Matthias Sachse, Jana Fassunke
      Pages 179-185

    9. Detection and Quantification of KIT Mutations in ctDNA by Plasma Safe-SeqS

      • Johannes Fredebohm, Daniel H. Mehnert, Ann-Kathrin Löber, Frank Holtrup, Vanessa van Rahden, Philipp Angenendt et al.
      Pages 187-189

  3. Ethics

    1. Front Matter

      Pages 191-191
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Keywords

About this book

The book will present the progress made since the last meeting in fall 2013 concerning the analysis of circulating extra-cellular nucleic acids. There are a modest number of laboratories involved in this field, nevertheless the number of papers published by researchers is extensive. The articles which will be published in this meeting report will be a valuable contribution for researchers and research students alike and will help them to stay on top of the developments in different research areas and to „cross borders“ between them.

Editors and Affiliations

  • ICRM, Inserm, Montpellier Cedex, France

    Peter B. Gahan

  • Universitätsklinikum Halle (Saale), Klinik für Innere Medizin, Schwerpunkt Pneumologie, Halle/Saale, Germany

    Michael Fleischhacker, Bernd Schmidt

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