Abstract
For the subsequent analysis of the methylated mSEPT9 colorectal cancer screening marker in plasma, different blood collection tubes and blood storage conditions were investigated. The study demonstrated that methylated Septin 9 (mSEPT9) can be consistently detected in plasma samples derived from whole blood samples collected with S-Monovette® K3E and BD Vacutainer ® K2EDTA tubes stored at 2–8 °C for a maximum of 24 h and for samples collected in S-Monovette CPDA tubes stored at 18–25 °C for up to 48 h.
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deVos T, Tetzner R, Model F et al (2009) Circulating methylated SEPT9 DNA in plasma is a biomarker for colorectal cancer. Clin Chem 55(7):1337–1346
Epigenomics AG (2014) IFU Epi proColon 2.0 CE. http://www.epiprocolon.com/en/e-support-library/instructions-for-use
Jin P, Kang Q, Wang X et al (2015) Performance of a second-generation methylated SEPT9 test in detecting colorectal neoplasm. J Gastroenterol Hepatol 30(5):830–833
Tóth K, Wasserkort R, Sipos et al (2014) Detection of methylated septin 9 in tissue and plasma of colorectal patients with neoplasia and the relationship to the amount of circulating cell-free DNA. PLoS One 19;9(12):e115415
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The authors were employees of Epigenomics AG. Juergen Distler and Thomas Koenig are also share holders of the Epigenomics AG.
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Distler, J., Tetzner, R., Weiss, G., König, T., Schlegel, A., Bagrowski, M. (2016). Evaluation of Different Blood Collection Tubes and Blood Storage Conditions for the Preservation and Stability of Cell-Free Circulating DNA for the Analysis of the Methylated mSEPT9 Colorectal Cancer Screening Marker. In: Gahan, P., Fleischhacker, M., Schmidt, B. (eds) Circulating Nucleic Acids in Serum and Plasma – CNAPS IX. Advances in Experimental Medicine and Biology, vol 924. Springer, Cham. https://doi.org/10.1007/978-3-319-42044-8_32
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DOI: https://doi.org/10.1007/978-3-319-42044-8_32
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Publisher Name: Springer, Cham
Print ISBN: 978-3-319-42042-4
Online ISBN: 978-3-319-42044-8
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