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Circulating Nucleic Acids in Serum and Plasma – CNAPS IX pp 133–136Cite as

Protein Content of Circulating Nucleoprotein Complexes

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 924))

Abstract

In the current study we have investigated the protein content of blood plasma deoxyribonucleoprotein complexes. The complexes were isolated using affinity chromatography with immobilized polyclonal anti-histone antibodies. Proteins were separated by SDS PAAGE and identified by MALDI-TOF mass-spectrometry. 111 and 56 proteins (excluding histones), respectively, were identified with a good score in deoxyribonucleoprotein complexes of healthy females and breast cancer patients. However, only four of these proteins were found in 30 % of all samples. Fourteen proteins previously described as tumor specific proteins were found in cancer patients whereas not one of them was found in healthy individuals. The data obtained demonstrate the involvement of different cellular and extracellular proteins in circulating cell-free DNA.

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References

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Conflicts of Interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev ave., 8, Novosibirsk, 630090, Russia

    Svetlana N. Tamkovich, Oleg S. Tutanov & Pavel P. Laktionov

  2. Lomonosov Moscow State University, Lomonosovsky ave., 31-5, Moscow, 11919, Russia

    Svetlana N. Tamkovich & Vsevolod A. Tkachuk

  3. Novosibirsk National Research State University, Pirogova str., 2, Novosibirsk, 630090, Russia

    Svetlana N. Tamkovich & Danil S. Serdukov

  4. Pirogov Russian National Research Medical University, Ostrovitianov str., 1, Moscow, Russia

    Maxim S. Belenikin

  5. Far Eastern Federal University, 8 Suhanova st., Vladivostok, Russia

    Anatoliy G. Shlikht

  6. Novosibirsk Regional Cancer Dispensary, Plakhotnogo str., 2, Novosibirsk, 630108, Russia

    Natalia A. Kirushina & Vladimir E. Voytsitskiy

  7. Institute “International Tomografic Center”, Institutskaya str., 3, Novosibirsk, Russia

    Yuri P. Tsentalovich

  8. Novosibirsk Research Institute of Circulation Pathology of Acadamician E.N. Meshalkin, Rechkunovskaya str., 15, Novosibirsk, 630090, Russia

    Pavel P. Laktionov

Authors
  1. Svetlana N. Tamkovich
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  2. Oleg S. Tutanov
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  3. Danil S. Serdukov
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  4. Maxim S. Belenikin
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  5. Anatoliy G. Shlikht
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  6. Natalia A. Kirushina
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  7. Vladimir E. Voytsitskiy
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  8. Yuri P. Tsentalovich
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  9. Vsevolod A. Tkachuk
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  10. Pavel P. Laktionov
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Corresponding author

Correspondence to Svetlana N. Tamkovich .

Editor information

Editors and Affiliations

  1. ICRM, Inserm, Montpellier Cedex, France

    Peter B. Gahan

  2. Universitätsklinikum Halle (Saale), Klinik für Innere Medizin, Schwerpunkt Pneumologie, Halle/Saale, Germany

    Michael Fleischhacker

  3. Universitätsklinikum Halle (Saale), Klinik für Innere Medizin, Schwerpunkt Pneumologie, Halle/Saale, Germany

    Bernd Schmidt

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© 2016 Springer International Publishing Switzerland

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Tamkovich, S.N. et al. (2016). Protein Content of Circulating Nucleoprotein Complexes. In: Gahan, P., Fleischhacker, M., Schmidt, B. (eds) Circulating Nucleic Acids in Serum and Plasma – CNAPS IX. Advances in Experimental Medicine and Biology, vol 924. Springer, Cham. https://doi.org/10.1007/978-3-319-42044-8_26

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