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Cell-free DNA as Diagnostic Markers

Methods and Protocols

  • Valentina Casadio
  • Samanta Salvi

Part of the Methods in Molecular Biology book series (MIMB, volume 1909)

Table of contents

  1. Front Matter
    Pages i-x
  2. An Overview

    1. Front Matter
      Pages 1-1
  3. A Liquid Biopsy for Cancer Disease

  4. Methods in Cancer

    1. Front Matter
      Pages 75-75
    2. Sara Ravaioli
      Pages 77-83
    3. Takashi Takeshita, Hirotaka Iwase
      Pages 105-118
    4. Michela Tebaldi, Samanta Salvi
      Pages 119-125
  5. Epigenetic Modifications

    1. Front Matter
      Pages 127-127
    2. Giorgia Gurioli
      Pages 129-135
    3. Luca Sigalotti, Alessia Covre, Francesca Colizzi, Elisabetta Fratta
      Pages 137-162
  6. Fetal and Pediatric Diseases

    1. Front Matter
      Pages 163-163
    2. Lucia Tombolan, Angelica Zin, Gianni Bisogno
      Pages 165-175
  7. Physical Activity

    1. Front Matter
      Pages 181-181
    2. Leydi Natalia Vittori, Andrea Tarozzi, Pasqualino Maietta Latessa
      Pages 183-197
  8. Urinary Cell Free DNA

    1. Front Matter
      Pages 199-199
    2. Samanta Salvi, Valentina Casadio
      Pages 201-209
    3. Valentina Casadio, Samanta Salvi
      Pages 211-221
  9. Back Matter
    Pages 223-224

About this book

Introduction

This book describes the most important techniques used for studying cfDNA in the different samples; serum, plasma, urine. Chapters detail methods on liquid biopsy for cancer disease, methods in cancer, epigenetic modifications, fetal and pediatric diseases, physical activity, and urinary cell free DNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Cell-free DNA as Diagnostic Markers: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Keywords

cancer Urinary cell free DNA Bioinformatics DNA integrity Mutations analyses

Editors and affiliations

  • Valentina Casadio
    • 1
  • Samanta Salvi
    • 2
  1. 1.Biosciences LaboratoryIRCCS I.R.S.TMeldolaItaly
  2. 2.Biosciences LaboratoryIRCCS I.R.S.TMeldolaItaly

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-8973-7
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8972-0
  • Online ISBN 978-1-4939-8973-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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