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A Guide to Protein Isolation

  • Textbook
  • © 2003
  • Latest edition

Overview

Authors:
  1. Clive Dennison

    1. School of Molecular and Cellular Biosciences, University of Natal, Pietermaritzburg, South Africa

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Part of the book series: Focus on Structural Biology (FOSB, volume 3)

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Table of contents (8 chapters)

  1. Front Matter

    Pages i-xvii
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  2. Basic physical concepts applicable to the isolation of proteins

    • Clive Dennison
    Pages 1-12

  3. An overview of protein isolation

    • Clive Dennison
    Pages 13-19

  4. Assay, extraction and subcellular fractionation

    • Clive Dennison
    Pages 20-57

  5. Concentration of the extract

    • Clive Dennison
    Pages 58-88

  6. Chromatography

    • Clive Dennison
    Pages 89-138

  7. Electrophoresis

    • Clive Dennison
    Pages 139-177

  8. Immunological methods

    • Clive Dennison
    Pages 178-209

  9. Some common practical methods

    • Clive Dennison
    Pages 210-227

  10. Back Matter

    Pages 228-249
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Keywords

About this book

It is a truism of science that the more fundamental the subject, the more universally applicable it is. Neverthelens, it is important to strike a level of "fundamentalness" appropriate to the task in hand. For example, an in-depth study of the mechanics of motor cars would tell one nothing about the dynamics of traffic. Traffic exists on a different "level" - it is dependent upon the existence of motor vehicles but the physics and mathematics of traffic can be adequately addressed by considering motor vehicles as mobile "blobs", with no consideration of how they become mobile. To start a discourse on traffic with a consideration of the mechanics of motor vehicles would thus be inappropropriate. In writing this volume, I have wrestled with the question of the appropriate level at which to address the physics underlying many of the techniques used in protein isolation. I have tried to strike a level as would be used by a mechanic (with perhaps a slight leaning towards an engineer) - i.e. a practical level, offering appropriate insight but with minimal mathematics. Some people involved in biochemical research have a minimal grounding in chemistry and physics and so I have tried to keep it as simple as possible.

Authors and Affiliations

  • School of Molecular and Cellular Biosciences, University of Natal, Pietermaritzburg, South Africa

    Clive Dennison

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