An overview of protein isolation

Abstract

Isolating a protein may be compared to playing a game of golf. In golf, the player is faced with a series of problems, each unique and yet similar to problems previously encountered. In facing each problem the player must analyse the situation and decide, from experience, which club is likely to give the best result in the given circumstances. Similarly, in attempting to isolate proteins, researchers face a series of similar-yetunique problems. To solve these they must dip into their bags and select an appropriate technique. The purpose of this book is thus to fill the beginner’s “golf bag” with techniques relevant to protein isolation, hopefully to improve their game.