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Table of contents (10 chapters)
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Front Matter
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Back Matter
Keywords
About this book
The advantages of obtaining a completely defined environment for the growth of cells in vitro were recognized very early in the history of cell culture (Lewis and Lewis, 1911). Continued interest in the nutritional requirements of cells in vitro and in providing an optimal environment for cells led to the development of the complex nutrient mixtures available today in many media (Waymouth, 1972; Ham, 1965). However, serum remained an essential component of medium for the growth of most cell types in culture. The question of what factor (or factors) in serum was essential for cell growth and survival remained unanswered for several decades. Initially, experiments were designed to purify the "active component" of serum for the growth of cells in culture. These experiments identified fetuin (Fisher et at., 1958) and nonsuppressible insulinlike activity (Temin et at., 1972) as important components of serum. However, the complexity of serum and the very low levels of active components in serum hindered progress in identi fying and isolating serum factors.
Editors and Affiliations
Bibliographic Information
Book Title: Mammalian Cell Culture
Book Subtitle: The Use of Serum-Free Hormone-Supplemented Media
Editors: Jennie P. Mather
DOI: https://doi.org/10.1007/978-1-4615-9361-4
Publisher: Springer New York, NY
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eBook Packages: Springer Book Archive
Copyright Information: Plenum Press, New York 1984
Softcover ISBN: 978-1-4615-9363-8Published: 31 January 2012
eBook ISBN: 978-1-4615-9361-4Published: 06 December 2012
Edition Number: 1
Number of Pages: XVIII, 284
Topics: Anatomy