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Kidney Cell Cultures in Hormonally Defined Serum-Free Medium

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Mammalian Cell Culture

Abstract

Renal cell growth and function may be studied in vitro using both established kidney cell lines and primary kidney cultures. To a major extent, in vitro studies concerning renal functions have been primarily concerned with two established kidney epithelial cell lines, Madin Darby canine kidney (MDCK) and the pig kidney cell line LLC-PK1. These two cell lines are well characterized with regard to their transport properties. However, in vitro studies concerning hormonal regulation of growth and transport have not been extensive. The hormonally defined, serum-free culture media for these cell lines should facilitate such studies. Furthermore, hormonally defined serum-free culture media should also prove to be invaluable in the study of primary kidney cultures.

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Authors and Affiliations

  1. Biochemistry Department, State University of New York at Buffalo, Buffalo, New York, 14214, USA

    Mary Taub

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  1. Mary Taub
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Editors and Affiliations

  1. The Population Council, New York, New York, USA

    Jennie P. Mather

  2. The Rockefeller University, New York, New York, USA

    Jennie P. Mather

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© 1984 Plenum Press, New York

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Taub, M. (1984). Kidney Cell Cultures in Hormonally Defined Serum-Free Medium. In: Mather, J.P. (eds) Mammalian Cell Culture. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9361-4_6

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  • DOI: https://doi.org/10.1007/978-1-4615-9361-4_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-9363-8

  • Online ISBN: 978-1-4615-9361-4

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