Fig. 5
Commonly used stains to assess renal fibrosis are shown: Fibrosis is visualized by different colors in each staining: Blue in trichrome, light pink/purple in PAS (vs. dark pink/purple for basement membranes), dark brown in collagen III immunohistochemistry, and red in picrosirius red stain . A glomerulus can be seen in the center of the images, and an area of fibrosis can be seen to the left of the glomerulus in each of the images (all at an original magnification of 200×)