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Automated Mass Fingerprinting of Venoms in Nanogram Range: Review of Technology

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Venom Genomics and Proteomics

Part of the book series: Toxinology ((TOXI))

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Abstract

Mass spectrometry (MS) is at present well recognized as a method of choice for the study of any venoms. As a most basic venomic analysis, venom mass fingerprinting (VMF) which depicts the venom’s all-inclusive masses may be very informative when comparisons among close or distant venomous species have to be pictured, as well as intraspecific venom variations. This chapter reports, as an example, the technological requirements and pitfalls using the state-of-the-art nano-ultrahigh-performance liquid chromatography (UHPLC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS in order to achieve in 2-h at the best and from tens of venom nanograms only a full-automated workflow. Lastly, having the opportunity of acquiring VMF in such a fast and easy way could be extremely valuable for carrying out phylogenetic studies based on mass clades.

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Authors and Affiliations

  1. Aix Marseille Université, CNRS, CRN2M, UMR7286, CS80011, bd Pierre Dramard, F-13344, 13015, Cedex 15, Marseille, France

    Pierre E. Bougis

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  1. Pierre E. Bougis
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Correspondence to Pierre E. Bougis .

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Editors and Affiliations

  1. Venom and Toxin Research Programme, Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

    P. Gopalakrishnakone

  2. Structural and Functional Venomics Lab, Instituto de Biomedicina de Valencia (CSIC), Valencia, Spain

    Juan J. Calvete

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Bougis, P.E. (2016). Automated Mass Fingerprinting of Venoms in Nanogram Range: Review of Technology. In: Gopalakrishnakone, P., Calvete, J. (eds) Venom Genomics and Proteomics. Toxinology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-6416-3_4

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