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Chemical Methods for Detection of Allergens and Skin Exposure

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Contact Dermatitis

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Abstract

Many allergens are widely used in both consumer and occupational products. In many cases, it is difficult to know all the ingredients of a product since most products are not sufficiently labelled. To diagnose and prevent allergic contact dermatitis, the demonstration of allergens in the products from the patient’s environment is important. Chemical analysis of a product can make it possible to demonstrate the presence or absence of known allergens. Simple spot tests or documented analytical methods such as thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), gas chromatography (GC), atomic absorption spectrophotometry (AAS), and inductively coupled plasma-mass spectrometry (ICP-MS) can be used. Moreover, with chemical methods, the purity of a substance can be checked and new allergens can be isolated and identified. Advanced methods such as mass spectrometry (MS), nuclear magnetic resonance spectroscopy (NMR), and infrared spectrophotometry (IR) are often required to identify isolated allergens.

Sigfrid Fregert deceased.

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Authors and Affiliations

  1. Department of Occupational and Environmental Dermatology, Skåne University Hospital, Malmö, Sweden

    Jakob Dahlin, Cecilia Svedman, Birgitta Gruvberger & Magnus Bruze

  2. Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden

    Carola Lidén

  3. Department of Occupational and Environmental Dermatology, Skåne University Hospital, Malmö, Sweden

    Sigfrid Fregert

Authors
  1. Jakob Dahlin
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  2. Cecilia Svedman
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  3. Birgitta Gruvberger
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  4. Magnus Bruze
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  5. Carola Lidén
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  6. Sigfrid Fregert
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Corresponding author

Correspondence to Cecilia Svedman .

Editor information

Editors and Affiliations

  1. Amtssygehuset Gentofte Dept. Dermatology, Hellerup, Denmark

    Jeanne Duus Johansen

  2. Federal Institute for Vaccines & Biomed, Paul Ehrlich Institut Federal Institute for Vaccines & Biomed, Langen, Bayern, Germany

    Vera Mahler

  3. Institut le Bel Labo. Dermatochimie, Strasbourg CX, France

    Jean-Pierre Lepoittevin

  4. Hautklinik, Städtische Kliniken Dortmund Klinikzentrum Mitte, Dortmund, Germany

    Peter J. Frosch

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Dahlin, J., Svedman, C., Gruvberger, B., Bruze, M., Lidén, C., Fregert, S. (2020). Chemical Methods for Detection of Allergens and Skin Exposure. In: Johansen, J., Mahler, V., Lepoittevin, JP., Frosch, P. (eds) Contact Dermatitis. Springer, Cham. https://doi.org/10.1007/978-3-319-72451-5_27-2

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  • DOI: https://doi.org/10.1007/978-3-319-72451-5_27-2

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  1. Latest

    Chemical Methods for Detection of Allergens and Skin Exposure
    Published:
    15 February 2020

    DOI: https://doi.org/10.1007/978-3-319-72451-5_27-2

  2. Original

    Chemical Methods for Detection of Allergens and Skin Exposure
    Published:
    13 November 2019

    DOI: https://doi.org/10.1007/978-3-319-72451-5_27-1

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