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Enhanced Secondary Metabolite Production in Hairy Root Cultures Through Biotic and Abiotic Elicitors

Plant Cell and Tissue Differentiation and Secondary Metabolites

Part of the book series: Reference Series in Phytochemistry ((RSP))

Abstract

Natural products from plants are of major pharmaceutical and therapeutic importance, several of which are often obtained from the underground parts of the concerned plants, necessitating the uprooting and killing of the whole plants. The biosynthetic capacity of “hairy roots” has appeared to closely mirror that of the roots of the parent plant from which they are derived and therefore has extensively been studied as an effective alternate system for in vitro secondary metabolite production. The growth rates and secondary metabolite yields of such transformed roots have also been improved by adopting several measures, such as: by alterations in nutrient composition, carbohydrate source and their levels, pH changes, supplementation with growth hormones, or elicitation with biotic or abiotic elicitors. Elicitors act by working as signals which are received by some elicitor-specific receptors present on the plant membranes, thus activating the defense responses which in turn lead to targeted secondary metabolite production. The present chapter discusses the effectiveness of different sources of elicitors (biotic or abiotic), their concentrations, and the time of application during hairy roots growth. The succeeding discussion in the chapter relates the present findings related to the genetically transformed “hairy root” cultures to the existing status of work on elicitation in medicinally important plant species for the maximum biomass and secondary metabolite production.

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Abbreviations

Ag+:

Silver

AgNO3:

Silver nitrate

AlCl3:

Aluminum chloride

ASA:

Acetylsalicylic acid

Ca:

Calcium

CaCl2:

Calcium chloride

CdCl2:

Cadmium chloride

Co:

Cobalt

CoCl2:

Cobalt chloride

Cor:

Coronatine

Cu:

Copper

CuCl2:

Copper chloride

CuSO4:

Copper sulfate

Fe:

Iron

H2O2:

Hydrogen peroxide

HgCl2:

Mercuric chloride

KCl:

Potassium chloride

Mg:

Magnesium

MeJA:

Methyl jasmonate

Mn:

Manganese

NaCl:

Sodium chloride

NO:

Nitric oxide

PEG:

Polyethylene glycol

SA:

Salicylic acid

SNP:

Sodium nitroprusside Terpenoid indole alkaloid (TIA)

UV:

Ultraviolet

VdSO4:

Vanedium sulfate

Zn:

Zinc

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Authors and Affiliations

  1. Institute of Bioscience and Technology, Shri Ramswaroop Memorial University, Barabanki, Uttar Pradesh, India

    Gurminder Kaur

  2. Molecular Biology and Biotechnology Division, Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Lucknow, Uttar Pradesh, India

    Pravin Prakash, Rakesh Srivastava & Praveen Chandra Verma

  3. Academy of Scientific and Innovative Research (AcSIR), Council of Scientific and Industrial Research, Ghaziabad, Uttar Pradesh, India

    Praveen Chandra Verma

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  4. Praveen Chandra Verma
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  1. Department of Botany, M.L.Sukhadia university, Udaipur, Rajasthan, India

    Kishan Gopal Ramawat

  2. Department of Pharmaceutical Botany, Jagiellonian University, Medical College, Kraków, Poland

    Halina Maria Ekiert

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Kaur, G., Prakash, P., Srivastava, R., Verma, P.C. (2020). Enhanced Secondary Metabolite Production in Hairy Root Cultures Through Biotic and Abiotic Elicitors. In: Ramawat, K., Ekiert, H., Goyal, S. (eds) Plant Cell and Tissue Differentiation and Secondary Metabolites. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-11253-0_38-1

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  • DOI: https://doi.org/10.1007/978-3-030-11253-0_38-1

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    Enhanced Secondary Metabolite Production in Hairy Root Cultures Through Biotic and Abiotic Elicitors
    Published:
    19 April 2023

    DOI: https://doi.org/10.1007/978-3-030-11253-0_38-2

  2. Original

    Enhanced Secondary Metabolite Production in Hairy Root Cultures Through Biotic and Abiotic Elicitors
    Published:
    11 August 2020

    DOI: https://doi.org/10.1007/978-3-030-11253-0_38-1

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