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Regulatory RNAs

Future Perspectives in Diagnosis, Prognosis, and Individualized Therapy

  • Protocol
Target Discovery and Validation Reviews and Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 361))

Abstract

With potentially up to 1000 microRNAs (miRNAs) present in the human genome, altogether regulating the expression of thousands of genes, one can anticipate that miRNAs will play a significant role in health and disease. Deregulated protein expression induced by a dysfunctional miRNA-based regulatory system is thus expected to lead to the development of serious, if not lethal, genetic diseases. A relationship among miRNAs, Dicer, and cancer has recently been suggested. Further investigations will help establish specific causal links between dysfunctional miRNAs and diseases. miRNAs of foreign origin, e.g., viruses, may also be used as specific markers of viral infections. In these cases, miRNA expression profiles could represent a powerful diagnostic tool. Regulatory RNAs may also have therapeutic applications, by which disease-causing genes or viral miRNAs could be neutralized, or functional miRNAs be restored. Will bedside miRNA expression profiling eventually assist physicians in providing patients with accurate diagnosis, personalized therapy, and treatment outcome?

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Authors and Affiliations

  1. Centre de Recherche en Rhumatologie et Immunology, Université Laval, Québec, Canada

    Marjorie P. Perron, Vincent Boissonneault, Lise-Andrée Gobeil, Dominique L. Ouellet & Patrick Provost

  2. Department of Anatomy and Physiology, Université Laval, Québec, Canada

    Patrick Provost

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  1. Marjorie P. Perron
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  2. Vincent Boissonneault
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  3. Lise-Andrée Gobeil
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  4. Dominique L. Ouellet
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  5. Patrick Provost
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Editors and Affiliations

  1. Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Oslo, Norway

    Mouldy Sioud

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Perron, M.P., Boissonneault, V., Gobeil, LA., Ouellet, D.L., Provost, P. (2007). Regulatory RNAs. In: Sioud, M. (eds) Target Discovery and Validation Reviews and Protocols. Methods in Molecular Biology™, vol 361. Humana Press. https://doi.org/10.1385/1-59745-208-4:311

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  • DOI: https://doi.org/10.1385/1-59745-208-4:311

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-890-4

  • Online ISBN: 978-1-59745-208-3

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