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Bioinformatics Approaches to Cancer Gene Discovery

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Target Discovery and Validation Reviews and Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 360))

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Summary

The Cancer Gene Anatomy Project (CGAP) database of the National Cancer Institute has thousands of known and novel expressed sequence tags (ESTs). These ESTs, derived from diverse normal and tumor cDNA libraries, offer an attractive starting point for cancer gene discovery. Data-mining the CGAP database led to the identification of ESTs that were predicted to be specific to select solid tumors. Two genes from these efforts were taken to proof of concept for diagnostic and therapeutics indications of cancer. Microarray technology was used in conjunction with bioinformatics to understand the mechanism of one of the targets discovered. These efforts provide an example of gene discovery by using bioinformatics approaches. The strengths and weaknesses of this approach are discussed in this review.

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Acknowledgments

I thank members of my laboratory for valuable contributions and Jeanine Narayanan for editorial assistance.

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Authors and Affiliations

  1. Center for Molecular Biology and Biotechnology and Department of Biology, Florida Atlantic University, Boca Raton, FL

    Ramaswamy Narayanan

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  1. Ramaswamy Narayanan
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Editors and Affiliations

  1. Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Oslo, Norway

    Mouldy Sioud

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© 2007 Humana Press Inc.

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Narayanan, R. (2007). Bioinformatics Approaches to Cancer Gene Discovery. In: Sioud, M. (eds) Target Discovery and Validation Reviews and Protocols. Methods in Molecular Biology™, vol 360. Humana Press. https://doi.org/10.1385/1-59745-165-7:13

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  • DOI: https://doi.org/10.1385/1-59745-165-7:13

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-656-6

  • Online ISBN: 978-1-59745-165-9

  • eBook Packages: Springer Protocols

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