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Target Discovery and Validation Reviews and Protocols

Emerging Strategies for Targets and Biomarker Discovery, Volume 1

  • Book
  • © 2007

Overview

Editors:
  1. Mouldy Sioud

    1. Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Oslo, Norway

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 360)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Main Approaches to Target Discovery and Validation

    • Mouldy Sioud
    Pages 1-12

  3. Bioinformatics Approaches to Cancer Gene Discovery

    • Ramaswamy Narayanan
    Pages 13-31

  4. Analysis of Gene Networks for Drug Target Discovery and Validation

    • Seiya Imoto, Yoshinori Tamada, Christopher J. Savoie, Satoru Miyanoaa
    Pages 33-56

  5. Target Discovery and Validation in Pancreatic Cancer

    • Robert M. Beaty, Mads Gronborg, Jonathan R. Pollack, Anirban Maitra
    Pages 57-89

  6. Molecular Classification of Breast Tumors

    • Therese Sørlie
    Pages 91-114

  7. Discovery of Differentially Expressed Genes

    • Øystein Røsok, Mouldy Sioud
    Pages 115-129

  8. Genome-Wide Screening by Using Small-Interfering RNA-Expression Libraries

    • Sahohime Matsumoto, Makoto Miyagishi, Kazunari Taira
    Pages 131-142

  9. Hammerhead Ribozyme-Based Target Discovery

    • Masayuki Sano, Kazunari Taira
    Pages 143-153

  10. Production of siRNA and cDNA-Transfected Cell Arrays on Noncoated Chambered Coverglass for High-Content Screening Microscopy in Living Cells

    • Holger Erfle, Rainer Pepperkok
    Pages 155-161

  11. Transgenic Animal Models in Biomedical Research

    • Louis-Marie Houdebine
    Pages 163-202

  12. Keratin Transgenic and Knockout Mice

    • Preethi Vijayaraj, Goran Söhl, Thomas M. Magin
    Pages 203-251

  13. The HUVEC/Matrigel Assay

    • Dag K. Skovseth, Axel M. Küchler, Guttorm Haraldsen
    Pages 253-268

  14. A Murine Model for Studying Hematopoiesis and Immunity in Heart Failure

    • Per Ole Iversen, Dag R. Sørensen
    Pages 269-275

  15. An Overview of the Immune System and Technical Advances in Tumor Antigen Discovery and Validation

    • Mouldy Sioud
    Pages 277-318

  16. Potential Target Antigens for Immunotherapy Identified by Serological Expression Cloning (SEREX)

    • Dirk Jäger
    Pages 319-326

  17. Identification of Tumor Antigens by Using Proteomics

    • François Le Naour
    Pages 327-334

  18. Protein Arrays

    • Lina Cekaite, Eivind Hovig, Mouldy Sioud
    Pages 335-348

  19. Back Matter

    Pages 349-354
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Keywords

About this book

Target discovery is a field that has existed for several years but is so vibrant today because of the recent progress in our understanding of the molecular mechanisms of many human diseases and the technical advances in target identification and validation. More sophisticated gene profiling technologies, such as DNA microarrays and serial analysis of gene expression, permit rapid identification of lead targets. Moreover, analysis of gene networks in living organisms allows the identification of target genes that operate in defined physiological pathways. With the sequencing of several genomes completed and the rapidly growing gene expression databases, there is now greater impetus than ever before for in silico discovery of therapeutic targets. Also, recent advances in genetic technologies have increased our ability to generate mouse models for human diseases. The implications of these genetically modified animals in drug development are several, including identification of new drug targets, predicting efficacy, and uncovering possible side effects. Together, these recent technical advances should allow researchers to make the most informed choice early and advance the chosen targets toward clinical studies. Regarding cancers, any difference between a cancer and a normal cell could potentially be exploited as a therapeutic target. The hope is that drugs targeting specific constituents or pathways in cancer cells will provide more effective therapy, either alone or in combination with other currently used anticancer drugs. In addition to drug targets, identifying new target antigens remains as much of a challenge as improving tumor vaccines already in the clinic.

Reviews

From the reviews:

"Volume 1 focuses on novel and innovative techniques, and presents the most up-to-date protocols available for maximizing the likelihood of achieving target-selective inhibition in vivo while minimizing side effects. … Cancer researchers will find tremendous value in the molecular classification of breast cancers and the review of protocols for tumor antigens and cancer vaccines. … provide a thorough review of the most cutting-edge methods available for each step in drug target identification, validation, and clinical application." (Anticancer Research, Vol. 27, May, 2007)

"Review current methods for drug target discovery and validation. … Volume 1 is focused on drug target and biomarker discovery. … the chapters are generally well written and clear, and provide extensive references to the current literature. … Overall … provide useful overviews that would provide entry into this field. Novice and more experienced researchers … would find useful information … ." (Jonathan B. Chaires, ChemMedChem, Issue 3, 2008)

Editors and Affiliations

  • Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Oslo, Norway

    Mouldy Sioud

Bibliographic Information

  • Book Title: Target Discovery and Validation Reviews and Protocols

  • Book Subtitle: Emerging Strategies for Targets and Biomarker Discovery, Volume 1

  • Editors: Mouldy Sioud

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1597451657

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-656-6Published: 15 October 2006

  • Softcover ISBN: 978-1-61737-699-3Published: 19 November 2010

  • eBook ISBN: 978-1-59745-165-9Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 354

  • Number of Illustrations: 86 b/w illustrations, 3 illustrations in colour

  • Topics: Oncology

  • Industry Sectors: Biotechnology, Health & Hospitals, Pharma

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