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Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery

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MicroRNA Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 342))

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Abstract

Conditional gene targeting is often a useful approach to elucidate the in vivo function of a gene. We use this approach to investigate the biological role of the RNA interference (RNAi) pathway in mammals. In addition, the RNAi machinery in mammalian cells can be exploited for gene knock-down experiments. In this chapter, we discuss the variety of experiments that can be performed using genetically engineered embryonic stem (ES) cells. ES cells provide a mammalian genetic system that is physiological, and tractable for mutagenesis and experimentation. This approach is economical and rapid, because it does not require production and breeding of genetically engineered mice.

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Author information

Authors and Affiliations

  1. The CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, Boston, MA

    Stefan A. Muljo

  2. The Dana-Farber Cancer Institute, Department of Cancer Biology, Harvard Medical School, Boston, MA

    Chryssa Kanellopoulou

Authors
  1. Stefan A. Muljo
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  2. Chryssa Kanellopoulou
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Editor information

Editors and Affiliations

  1. Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

    Shao-Yao Ying

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© 2006 Humana Press Inc.

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Muljo, S.A., Kanellopoulou, C. (2006). Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery. In: Ying, SY. (eds) MicroRNA Protocols. Methods in Molecular Biology™, vol 342. Humana Press. https://doi.org/10.1385/1-59745-123-1:57

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  • DOI: https://doi.org/10.1385/1-59745-123-1:57

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-581-1

  • Online ISBN: 978-1-59745-123-9

  • eBook Packages: Springer Protocols

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