MicroRNA Protocols

  • Shao-Yao Ying

Part of the Methods in Molecular Biology™ book series (MIMB, volume 342)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Shao-Yao Ying, Donald C. Chang, Joseph D. Miller, Shi-Lung Lin
    Pages 1-18
  3. Jacek Krol, Wlodzimierz J. Krzyzosiak
    Pages 19-32
  4. Richard I. Gregory, Thimmaiah P. Chendrimada, Ramin Shiekhattar
    Pages 33-47
  5. Yan Zeng, Bryan R. Cullen
    Pages 49-56
  6. Julia A. Chekanova, Dmitry A. Belostotsky
    Pages 73-85
  7. Marc Rehmsmeier
    Pages 87-99
  8. Bino John, Chris Sander, Debora S. Marks
    Pages 101-113
  9. Neil R. Smalheiser, Vetle I. Torvik
    Pages 115-127
  10. Sam Griffiths-Jones
    Pages 129-138
  11. Michael Z. Michael
    Pages 189-207
  12. Chun-Hong Chen, Ming Guo, Bruce A. Hay
    Pages 229-240
  13. Yamina Bennasser, Shu-Yun Le, Man Lung Yeung, Kuan-Teh Jeang
    Pages 241-253
  14. Shinya Omoto, Yoichi R. Fujii
    Pages 255-265

About this book


It is now known that microRNA (miRNA), once thought to be junk, can suppress the expression of other genes and may be involved in numerous cellular processes, including cell proliferation, apoptosis, cell differentiation, and development. In MicroRNA Protocols, expert miRNA researchers explain its basic concepts and introduce the most advanced technologies and techniques for predicting screening, isolating, and assaying it. The authors provide diverse, novel, and useful descriptions of miRNAs in several species-including plants, worms, flies, fish, chicks, mice, and humans-and show how they have been employed to develop miRNA as a potential drug design tool. Highlights include miRNA and their target prediction by computer analysis, functional assays of miRNA in vitro and in vivo, and transgenic animal models using miRNA technologies. Additional chapters cover gene regulation, small RNA function, RNA interference mechanisms, and transgenetics. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Eminently practical and cutting-edge, MicroRNA Protocols affords biomedical researchers readily reproducible techniques to understand and study the molecular pathogenesis of disease and design new therapeutic strategies.


Vivo evolution genes hybridization miRNAs tissue

Editors and affiliations

  • Shao-Yao Ying
    • 1
  1. 1.Department of Cell and NeurobiologyKeck School of Medicine, University of Southern CaliforniaLos AngelesUSA

Bibliographic information

  • DOI https://doi.org/10.1385/1597451231
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-581-1
  • Online ISBN 978-1-59745-123-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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