Skip to main content

Perspectives

  • Protocol
MicroRNA Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 342))

Abstract

The discoveries of microRNAs (miRNAs), a class of noncoding RNAs that can regulate gene expression by translational repression, have opened a new avenue on gene modulation in mammalian cells. Conceivably, this highly effective method of modulation of gene expression would be exploited for numerous prospectives, including human, therapeutics. This chapter offers some perspective, with emphasis on areas that can be further developed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Wood, M. J., Trulzsch, B., Abdelgany, A., and Beeson, D. (2003) Therapeutic gene silencing in the nervous system. Hum. Mol. Genet. Spec No 2, R279–R284.

    Google Scholar 

  2. Davidson, B. L. and Paulson, H. L. (2004) Molecular medicine for the brain: silencing of disease genes with RNA interference. Lancet Neurol. 3, 145–149.

    Article  CAS  PubMed  Google Scholar 

  3. Siomi, H., Ishizuka, A., and Siomi, M. C. (2004) RNA interference: a new mechanism by which FMRP acts in the normal brain? What can Drosophila teach us? Ment. Retard. Dev. Disabil. Res. Rev. 10, 68–74.

    Article  PubMed  Google Scholar 

  4. Roll-Mecak, A. and Vale, R. D. (2005) The Drosophila homologue of the hereditary spastic paraplegia protein, spastin, severs and disassembles microtubules. Curr. Biol. 15, 650–655.

    Article  CAS  PubMed  Google Scholar 

  5. Crabbe, J. C. (2001) Use of genetic analyses to refine phenotypes related to alcohol tolerance and dependence. Alcohol Clin. Exp. Res. 25, 288–292.

    Article  CAS  PubMed  Google Scholar 

  6. Bahi, A., Boyer, F., Kolira, M., and Dreyer, J. L. (2005) In vivo gene silencing of CD81 by lentiviral expression of small interference RNAs suppresses cocaine-induced behaviour. J. Neurochem. 92, 1243–1255.

    Article  CAS  PubMed  Google Scholar 

  7. Nair, M. P., Schwartz, S. A., Mahajan, S. D., et al. (2004) Drug abuse and neuropathogenesis of HIV infection: role of DC-SIGN and IDO. J. Neuroimmunol. 157, 56–60.

    Article  CAS  PubMed  Google Scholar 

  8. Calin, G. A., Dumitru, C. D., Shimizu, M., et al. (2002) Frequent deletions and downregulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia. Proc. Natl. Acad. Sci. USA 99, 15,524–15,529.

    Article  CAS  PubMed  Google Scholar 

  9. Michael, M. Z., O’Connor, S. M., van Holst Pellekaan, N. G., Young, G. P., and James, R. J. (2003) Reduced accumulation specific microRNAs in colorectal neoplasia. Mol. Cancer Res. 1, 882–891.

    CAS  PubMed  Google Scholar 

  10. Takamizawa, J., Konishi, H., Yanagisawa, K., et al. (2004) Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival. Cancer Res. 64, 3753–3756.

    Article  CAS  PubMed  Google Scholar 

  11. Metzler, M., Wilda, M., Busch, K., Viehmann, S., and Borkhardt, A. (2004) High expression of precursor microRNA-155/BIC RNA in children with Burkitt lymphoma. Genes Chromosomes Cancer 39, 167–169.

    Article  CAS  PubMed  Google Scholar 

  12. Calin, G. A., Sevignani, C., Dumitru, C. D., et al. (2004) Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc. Natl. Acad. Sci. USA 101, 2999–3004.

    Article  CAS  PubMed  Google Scholar 

  13. Olsson, T., Piehl, F., Swanberg, M., and Lidman, O. (2005) Genetic dissection of neurodegeneration and CNS inflammation. J. Neurol. Sci. 233, 99–108.

    Article  CAS  PubMed  Google Scholar 

  14. Vitkovic, L., Maeda, S., and Sternberg, E. (2001) Anti-inflammatory cytokines: expression and action in the brain. Neuroimmunomodulation 9, 295–312.

    Article  CAS  PubMed  Google Scholar 

  15. Boven, L. A., Middel, J., Portegies, P., Verhoef, J., Jansen, G. H., and Nottet, H. S. (1999) Overexpression of nerve growth factor and basic fibroblast growth factor in AIDS dementia complex. J. Neuroimmunol. 97, 154–162.

    Article  CAS  PubMed  Google Scholar 

  16. Richardson, M. K. (1995) Heterochrony and the phylotypic period. Dev. Biol. 172, 412–421.

    Article  CAS  PubMed  Google Scholar 

  17. Lonai, P. and Orr-Urtreger, A. (1990) Homeogenes in mammalian development and the evolution of the cranium and central nervous system. FASEB J. 4, 1436–1443.

    CAS  PubMed  Google Scholar 

  18. Hall, B. K. (2003) Evo-Devo: evolutionary developmental mechanisms. Int. J. Dev. Biol. 47, 491–495.

    PubMed  Google Scholar 

  19. Case, C. C. (2003) Transcriptional tools for aging research. Mech. Ageing Dev. 124, 103–108.

    Article  CAS  PubMed  Google Scholar 

  20. Deocaris, C. C., Kaul, S. C., Taira, K., and Wadhwa, R. (2004) Emerging technologies: trendy RNA tools for aging research. J. Gerontol. A. Biol. Sci. Med. Sci. 59, 771–783.

    PubMed  Google Scholar 

  21. Coffer, P. (2003) OutFOXing the grim reaper: novel mechanisms regulating longevity by forkhead transcription factors. Sci. STKE 2003, PE39.

    Article  PubMed  Google Scholar 

  22. Kirkwood, T. B. (2003) Genes that shape the course of ageing. Trends Endocrinol. Metab. 14, 345–347.

    Article  CAS  PubMed  Google Scholar 

  23. Luo, Y. (2004) Long-lived worms and aging. Redox Rep. 9, 65–69.

    Article  PubMed  Google Scholar 

  24. Morley, J. E., Mooradian, A. D., Silver, A. J., Heber, D., and Alfin-Slater, R. B. (1988) Nutrition in the elderly. Ann. Intern. Med. 109, 890–904.

    CAS  PubMed  Google Scholar 

  25. Nelson, J. F., Karelus, K., Bergman, M. D., and Felicio, L. S. (1995) Neuroendocrine involvement in aging: evidence from studies of reproductive aging and caloric restriction. Neurobiol. Aging 16, 837–843.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2006 Humana Press Inc.

About this protocol

Cite this protocol

Ying, SY., Chang, D.C., Miller, J.D., Lin, SL. (2006). Perspectives. In: Ying, SY. (eds) MicroRNA Protocols. Methods in Molecular Biology™, vol 342. Humana Press. https://doi.org/10.1385/1-59745-123-1:351

Download citation

  • DOI: https://doi.org/10.1385/1-59745-123-1:351

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-581-1

  • Online ISBN: 978-1-59745-123-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics