Abstract
In situ amplification techniques are designed to increase the mass of DNA in a fixed target, either whole cells or tissue sections. When combined with fluorescently labeled nucleotides, they can be used for locus detection. They also can be used to increase target mass for subsequent operations, such as cellular or chromosomal isolation by microdissection. When combined with chromosome microdissection, these techniques allow libraries to be made from single copies of chromosomes, chromosome fragments, or even bacteria.
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Acknowledgment
This work was performed under the auspices of the US Department of Energy by University of California, Lawrence Livermore National Laboratory under Contract W-7405-Eng-48.
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© 2006 Humana Press Inc.
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Christian, A.T., Hara, C. (2006). Microdissection With PCR In Situ . In: Pellestor, F. (eds) PRINS and In Situ PCR Protocols. Methods in Molecular Biology™, vol 334. Humana Press. https://doi.org/10.1385/1-59745-068-5:161
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DOI: https://doi.org/10.1385/1-59745-068-5:161
Publisher Name: Humana Press
Print ISBN: 978-1-58829-549-1
Online ISBN: 978-1-59745-068-3
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