PRINS and In Situ PCR Protocols

  • Franck Pellestor

Part of the Methods in Molecular Biology™ book series (MIMB, volume 334)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Basic Methodology

    1. Front Matter
      Pages 1-1
    2. Ju Yan, Macoura Gadji, Kada Krabchi, Régen Drouin
      Pages 3-13
    3. Franck Pellestor, Petra Paulasova
      Pages 15-22
    4. Stephen S. Wachtel, Avirachan T. Tharapel
      Pages 33-40
    5. Franck Pellestor, Brigitte Andréo, Jacques Puechberty, Geneviève Lefort, Pierre Sarda
      Pages 49-59
    6. Omar Bagasra, Twaina Harris
      Pages 61-78
  3. Research and Clinical Applications

    1. Front Matter
      Pages 79-79
    2. Avirachan T. Tharapel, Stephen S. Wachtel
      Pages 105-113
    3. Liborio Stuppia, Dario La Sala, Caterina Cinti
      Pages 115-122
    4. Ivan Y. Iourov, Svetlana G. Vorsanova, Franck Pellestor, Yuri B. Yurov
      Pages 123-132
    5. Jirí Macas, Alice Navrátilová, Marie Kubaláková, Jaroslav Dolezel
      Pages 133-139
    6. Kada Krabchi, Macoura Gadji, Ju Yan, Régen Drouin
      Pages 141-149
    7. Franck Pellestor, Brigitte Andréo, Tal Anahory, Samir Hamamah
      Pages 151-160
    8. Allen T. Christian, Christine Hara
      Pages 161-168
    9. Tamotsu Takeuchi, Yoshihiro Adachi, Hiroshi Sonobe, Yuji Ohtsuki
      Pages 169-179

About this book

Introduction

Carrying on the high standards of the much-praised first edition, leading researchers and clinical investigators have completely revised and updated PRINS and In Situ PCR Protocols to take advantage of the many new developments and applications that have occurred for cellular and molecular investigations. The authors contribute proven protocols for many variations of PRINS and in situ PCR, including a new, fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutations, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. They describe, in detail, various applications of PRINS in human cells (detection of gene deletions in cancer, detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as in plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, PRINS and In Situ PCR Protocols offers molecular biologists, cytogeneticists, and cytologists a cutting-edge guide to today's new in situ labeling technology.

Keywords

DNA Mutation PCR chromosome genes hybridization transcription

Editors and affiliations

  • Franck Pellestor
    • 1
  1. 1.Institute of Human GeneticsMontpellierFrance

Bibliographic information

  • DOI https://doi.org/10.1385/1597450685
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-549-1
  • Online ISBN 978-1-59745-068-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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