Abstract
An ultrarapid three- and four-color primed in situ (PRINS) procedure has been developed for rapid chromosome identification and aneuploidy assessment on isolated cells. Based on the direct in situ mixing of fluorochromes (fluorescein isothiocyanate, tetramethylrhodamine isothiocyanate, Cascade Blue), this multicolor PRINS procedure is described on unfertilized human oocytes and isolated human blastomeres.
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References
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Acknowledgment
The experiments performed for elaborating this protocol were supported by a European INTAS project no. 03-51-4060.
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© 2006 Humana Press Inc.
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Pellestor, F., Andréo, B., Anahory, T., Hamamah, S. (2006). PRINS as an Efficient Tool for Aneuploidy Assessment in Human Oocytes and Preimplantation Embryos. In: Pellestor, F. (eds) PRINS and In Situ PCR Protocols. Methods in Molecular Biology™, vol 334. Humana Press. https://doi.org/10.1385/1-59745-068-5:151
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DOI: https://doi.org/10.1385/1-59745-068-5:151
Publisher Name: Humana Press
Print ISBN: 978-1-58829-549-1
Online ISBN: 978-1-59745-068-3
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