Abstract
Both primed in situ labeling (PRINS) and peptide nucleic acid (PNA) technologies have emerged as research techniques, but they have quickly evolved to applications in biological diagnosis assays. The two procedures present several features (specificity, discriminating ability, rapidity) that make them very attractive for cytogenetic purposes. The combined use of PRINS and PNA for in situ chromosomal detection on a same cell preparation is described in this chapter.
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Acknowledgment
The experiments performed for elaborating this protocol were supported by the Projet Hospitalier de Recherche Clinique, a French research project (no. 7732), from the Centre Hospitalo-Universitaire of Montpellier and a European INTAS project no. 03-51-4060. P. Paulasova was supported by a Czech fellowship VZ FNM 00000064203.
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© 2006 Humana Press Inc.
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Pellestor, F., Paulasova, P. (2006). PRINS Combined With Peptide Nucleic Acid Labeling. In: Pellestor, F. (eds) PRINS and In Situ PCR Protocols. Methods in Molecular Biology™, vol 334. Humana Press. https://doi.org/10.1385/1-59745-068-5:15
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DOI: https://doi.org/10.1385/1-59745-068-5:15
Publisher Name: Humana Press
Print ISBN: 978-1-58829-549-1
Online ISBN: 978-1-59745-068-3
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