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Isolation of Rat Bone Marrow Stem Cells

  • Protocol
Cytochrome P450 Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 320))

Abstract

Stem cell research has become an important field of study for molecular, cellular, and clinical biology as well as pharmaco-toxicology. Indeed, stem cells have a strong proliferative and unlimited self-renewal potential and are multipotent. In vivo as well as in vitro studies have confirmed the differentiation of adult bone marrow stem cells into muscle cells, adipocytes, cardiomyocytes, neuroectodermal cells, osteoblasts, chondroblasts, and so on. Recently, it has been shown that, under appropriate culture conditions, adult bone marrow stem cells may also differentiate into hepatocyte-like cells. Because of their extensive proliferative capacity and pluripotency, adult bone marrow stem cells could serve in the future as an unlimited source of hepatocytes for pharmaco-toxicological research and testing. We describe a protocol for isolation of mononuclear cells from adult rat bone marrow.

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Acknowledgments

This work was supported by a PhD grant from the Institute for the Promotion of Innovation Through Science and Technology in Flanders (IWT-Vlaanderen) and grants from the Fund of Scientific Research Flanders (FWO), Belgium; and the Research Council of the Vrije Universiteit Brussel, Belgium.

Author information

Authors and Affiliations

  1. Department of Toxicology, Vrije Universiteit Brussel, Belgium

    Tamara Vanhaecke & Vera Rogiers

  2. Division of Molecular Toxicology, IMM, Karolinska Institutet, Stockholm, Sweden

    Sarah Snykers

Authors
  1. Sarah Snykers
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  2. Tamara Vanhaecke
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  3. Vera Rogiers
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Editor information

Editors and Affiliations

  1. School of Biological Sciences, Queen Mary University of London, London, UK

    Ian R. Phillips

  2. Department of Biochemistry and Molecular Biology, University College London, London, UK

    Elizabeth A. Shephard

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© 2006 Humana Press Inc.

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Snykers, S., Vanhaecke, T., Rogiers, V. (2006). Isolation of Rat Bone Marrow Stem Cells. In: Phillips, I.R., Shephard, E.A. (eds) Cytochrome P450 Protocols. Methods in Molecular Biology, vol 320. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-998-2:265

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  • DOI: https://doi.org/10.1385/1-59259-998-2:265

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-58829-441-8

  • Online ISBN: 978-1-59259-998-1

  • eBook Packages: Springer Protocols

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