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Cytochrome P450 Protocols

  • Book
  • © 2006

Overview

Editors:
  1. Ian R. Phillips

    1. School of Biological Sciences, Queen Mary University of London, London, UK

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  2. Elizabeth A. Shephard

    1. Department of Biochemistry and Molecular Biology, University College London, London, UK

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 320)

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Table of contents (37 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Cytochrome P450 Nomenclature, 2004

    • David R. Nelson
    Pages 1-10

  3. Spectral Analyses of Cytochromes P450

    • John B. Schenkman, Ingela Jansson
    Pages 11-18

  4. Establishment of Functional Human Cytochrome P450 Monooxygenase Systems in Escherichia coli

    • Michael P. Pritchard, Lesley McLaughlin, Thomas Friedberg
    Pages 19-29

  5. Purification of Cytochromes P450

    • F. Peter Guengerich, Martha V. Martin
    Pages 31-38

  6. Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System

    • Azara Janmohamed, Prapimpuk Thaunsukon, Elizabeth A. Shephard, Ian R. Phillips
    Pages 39-59

  7. Cytochrome P450 Reconstitution Systems

    • Hiroshi Yamazaki, Tsutomu Shimada
    Pages 61-71

  8. Catalytic Assays for Human Cytochrome P450

    • Thomas K. H. Chang, David J. Waxman
    Pages 73-83

  9. Enzymatic Analysis of cDNA-Expressed Human CYP1A1, CYP1A2, and CYP1B1 With 7-Ethoxyresorufin as Substrate

    • Thomas K. H. Chang, David J. Waxman
    Pages 85-90

  10. Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation

    • David J. Waxman, Thomas K. H. Chang
    Pages 91-96

  11. Determination of CYP2B6 Component of 7-Ethoxy-4-Trifluoromethylcoumarin O-Deethylation Activity in Human Liver Microsomes

    • Thomas K. H. Chang, Charles L. Crespi, David J. Waxman
    Pages 97-102

  12. High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6α-Hydroxylation

    • Charles L. Crespi, Thomas K. H. Chang, David J. Waxman
    Pages 103-107

  13. Determination of CYP2C9-Catalyzed Diclofenac 4′-Hydroxylation by High-Performance Liquid Chromatography

    • Charles L. Crespi, Thomas K. H. Chang, David J. Waxman
    Pages 109-113

  14. CYP2C19-Mediated (S)-Mephenytoin 4′-Hydroxylation Assayed by High-Performance Liquid Chromatography With Radiometric Detection

    • Charles L. Crespi, Thomas K. H. Chang, David J. Waxman
    Pages 115-119

  15. CYP2D6-Dependent Bufuralol 1′-Hydroxylation Assayed by Reverse-Phase Ion-Pair High-Performance Liquid Chromatography With Fluorescence Detection

    • Charles L. Crespi, Thomas K. H. Chang, David J. Waxman
    Pages 121-125

  16. Spectrophotometric Analysis of Human CYP2E1-Catalyzed p-Nitrophenol Hydroxylation

    • Thomas K. H. Chang, Charles L. Crespi, David J. Waxman
    Pages 127-131

  17. Thin-Layer Chromatography Analysis of Human CYP3A-Catalyzed Testosterone 6β-Hydroxylation

    • David J. Waxman, Thomas K. H. Chang
    Pages 133-141

  18. Determination of CYP4A11-Catalyzed Lauric Acid 12-Hydroxylation by High-Performance Liquid Chromatography With Radiometric Detection

    • Charles L. Crespi, Thomas K. H. Chang, David J. Waxman
    Pages 143-147

  19. An Isocratic High-Performance Liquid Chromatography Assay for CYP7A1-Catalyzed Cholesterol 7α-Hydroxylation

    • David J. Waxman, Thomas K. H. Chang
    Pages 149-152

  20. Use of 7-Ethoxycoumarin to Monitor Multiple Enzymes in the Human CYP1, CYP2, and CYP3 Families

    • David J. Waxman, Thomas K. H. Chang
    Pages 153-156
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Keywords

About this book

For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source.

Reviews

From Reviews of the First Edition...

"...a remarkable book, highly useful for all our scientific readers..." -Cellular and Molecular Biology

"...will be welcomed by laboratory scientists of various profiles who need authoritative protocols for their studies." -Modern Pathology

From the reviews of the second edition:

"In this second edition, editors Phillips and Shephard have updated the text to reflect current advances and to include chapters on some more contemporary procedures … . I’m sure that the wealth of background information in the book would be valuable. Overall … this is a useful reference book for experienced researchers and for those new to this discipline. Recommended." (David Wilkinson, British Toxicology Society Newsletter, Winter, 2006)

Editors and Affiliations

  • School of Biological Sciences, Queen Mary University of London, London, UK

    Ian R. Phillips

  • Department of Biochemistry and Molecular Biology, University College London, London, UK

    Elizabeth A. Shephard

Bibliographic Information

  • Book Title: Cytochrome P450 Protocols

  • Editors: Ian R. Phillips, Elizabeth A. Shephard

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592599982

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-441-8Published: 15 December 2005

  • Softcover ISBN: 978-1-61737-572-9Published: 19 November 2010

  • eBook ISBN: 978-1-59259-998-1Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 364

  • Number of Illustrations: 47 b/w illustrations

  • Topics: Biochemistry, general

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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