Abstract
During human pregnancy, fetal placental cells known as trophoblast invade into the uterine mucosal lining, coming into contact with maternal cells including a specialized population of leukocytes. In order to understand the interaction of maternal cells with trophoblast, it is useful to be able to isolate the various cell types from the fetal-maternal interface so that they may be characterized and co-cultured in vitro. This chapter details the methods we use in our laboratory for enrichment and/or purification of trophoblast cells, decidual leukocytes, decidual natural killer cells, decidual stromal cells, and decidual glandular epithelial cells from human first-trimester tissue samples.
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References
Boyd, J. D. and Hamilton, W. J. (1970) The Human Placenta. W. Heffer and Sons, Cambridge, UK.
Kam, E. P., Gardner, L., Loke, Y. W., and King, A. (1999) The role of trophoblast in the physiological change in decidual spiral arteries. Hum. Reprod. 14, 2131–2138.
Burrows, T. D., King, A., and Loke, Y. W. (1996) Trophoblast migration during human placental implantation. Hum. Reprod. Update 2, 307–321.
Loke, Y. W. and King, A. (1995) Human Implantation: Cell Biology and Immunology. Cambridge University Press, Cambridge.
King, A. (2000) Uterine leukocytes and decidualization. Hum. Reprod. Update 6, 28–36.
Trundley, A. and Moffett, A. (2004) Human uterine leukocytes and pregnancy. Tissue Antigens 63, 1–12.
Moffett-King, A. (2002) Natural killer cells and pregnancy. Nat. Rev. Immunol. 2, 656–663.
King, A., Thomas, L., and Bischof, P. (2000) Cell culture models of trophoblast II: trophoblast cell lines—a workshop report. Placenta 21(Suppl A), S113–S119.
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© 2006 Humana Press Inc.
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Trundley, A., Gardner, L., Northfield, J., Chang, C., Moffett, A. (2006). Methods for Isolation of Cells from the Human Fetal-Maternal Interface. In: Soares, M.J., Hunt, J.S. (eds) Placenta and Trophoblast. Methods in Molecular Medicine™, vol 122. Humana Press. https://doi.org/10.1385/1-59259-989-3:109
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DOI: https://doi.org/10.1385/1-59259-989-3:109
Publisher Name: Humana Press
Print ISBN: 978-1-58829-608-5
Online ISBN: 978-1-59259-989-9
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