Placenta and Trophoblast

Methods and Protocols Volume 2

  • Michael J. Soares
  • Joan S. Hunt

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 122)

Table of contents

  1. Front Matter
    Pages i-xix
  2. INTRODUCTION

    1. Front Matter
      Pages 1-1
    2. Michael J. Soares, Joan S. Hunt
      Pages 3-7
  3. Analysis of Trophoblast Invasion

    1. Front Matter
      Pages 9-9
    2. Robert Pijnenborg, Elizabeth Ball, Judith N. Bulmer, Myriam Hanssens, Stephen C. Robson, Lisbeth Vercruysse
      Pages 11-44
    3. John D. Aplin
      Pages 45-57
    4. Judith E. Cartwright, Mark Wareing
      Pages 59-74
  4. Analysis of Uteroplacental Immune Cells and Their Functions

    1. Front Matter
      Pages 75-75
    2. Thaddeus G. Golos, Gennadiy I. Bondarenko, Edith E. Breburda, Svetlana V. Dambaeva, Maureen Durning, Igor I. Slukvin
      Pages 93-108
    3. Anita Trundley, Lucy Gardner, Jacquie Northfield, Chiwen Chang, Ashley Moffett
      Pages 109-122
    4. Ramsey H. McIntire, Margaret G. Petroff, Teresa A. Phillips, Joan S. Hunt
      Pages 123-148
    5. Gil Mor, Shawn L. Straszewski-Chavez, Vikki M. Abrahams
      Pages 149-163
    6. Daudi K. Langat, Asgerally T. Fazleabas, Joan S. Hunt
      Pages 165-180
    7. Judith L. Pace, Pedro J. Morales, Joan S. Hunt, Teresa A. Phillips
      Pages 181-203
  5. Analysis of Placental Function

    1. Front Matter
      Pages 205-205
    2. Timothy R. H. Regnault, William W. Hay Jr.
      Pages 207-224
    3. Claudia J. Bode, Hong Jin, Erik Rytting, Peter S. Silverstein, Amber M. Young, Kenneth L. Audus
      Pages 225-239
    4. Jocelyn D. Glazier, Colin P. Sibley
      Pages 241-252
    5. Susan L. Greenwood, Colin P. Sibley
      Pages 253-264

About this book

Introduction

Placental research has progressed rapidly over the past several decades by taking advantage of technical advances, such as microarray analysis, reverse transcriptase polymerase chain reaction, protein analysis, and in situ hybridization. In Placenta and Trophoblast: Methods and Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation, and function. Volume 2 provides readily reproducible protocols for studying trophoblast invasion, maternal immune cell responses to pregnancy, and the hormone-producing capabilities of the placenta. There are also methods to investigate the barrier function of the placenta, its role in regulating the transport of nutrients and wastes, and the adaptations of trophoblast cells and the placenta to disease. Highlights include techniques to analyze trophoblast cell interactions with the uterine vasculature and immune cells, and to the endocrine functions of the placenta. A companion first volume concentrates on methods for investigate embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta.
Comprehensive and state-of-the-art, Placenta and Trophoblast: Methods and Protocols, Volumes 1 & 2 provide researchers a firm foundation for successful cellular and molecular analysis of the placenta and the establishment of pregnancy.

Keywords

Implantat cells development embryo endocrinology hormones placenta pregnancy regulation

Editors and affiliations

  • Michael J. Soares
    • 1
  • Joan S. Hunt
    • 2
  1. 1.Institute of Maternal-Fetal Biology Division of Cancer and Developmental Biology Department of Pathology and Laboratory MedicineUniversity of Kansas Medical CenterKansas City
  2. 2.Department of Anatomy and Cell BiologyUniversity of Kansas Medical Center

Bibliographic information

  • DOI https://doi.org/10.1385/1592599893
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-608-5
  • Online ISBN 978-1-59259-989-9
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • About this book
Industry Sectors
Biotechnology
Pharma