Abstract
In vitro models for human trophoblasts were initially established more than three decades ago from isolated choriocarcinomas. They have proven to be extremely valuable for the study of the cellular, molecular, and endocrine aspects of human trophoblasts. This chapter describes basic methods for culture and maintenance of the Jeg-3, Jar, and BeWo human choriocarcinoma cell lines as well as an effective paradigm for introducing DNA into the cells.
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Wolfe, M.W. (2006). Culture and Transfection of Human Choriocarcinoma Cells. In: Soares, M.J., Hunt, J.S. (eds) Placenta and Trophoblast. Methods in Molecular Medicineā¢, vol 121. Humana Press. https://doi.org/10.1385/1-59259-983-4:227
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DOI: https://doi.org/10.1385/1-59259-983-4:227
Publisher Name: Humana Press
Print ISBN: 978-1-58829-404-3
Online ISBN: 978-1-59259-983-7
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